FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4032426
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC395010997.95862341925184Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC279080.6920895758533449No Hit
TGTAGATA134410.33332291776712086No Hit
TGAAGATC64400.15970534859164187No Hit
AGTAGATC53540.13277367024218176No Hit
TGTAGAAC43730.10844588344584626Illumina DpnII expression Adapter 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)