FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30200406
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGG1057570.3501840339497423No Hit
CTTAGAGACAATCGCT321920.10659459346341238No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG456.949995E-410.00250110
CTCGCGC857.186827E-910.00250110
TTCGCCG553.89024E-510.00250110
GTCCGTA24000.010.0017399
CGGCCAA5000.010.0017389
ACTTCCG400.002954471410.0012578
ACCGGTT33700.010.0012568
ATGGTCG1200.010.0003787
CACGTCG456.9635606E-410.0003787
GGATTCG24450.010.0003787
CGGAGTT2300.010.0000646
CGTGGGC5600.010.0000636
ATTGTCA9950.010.0000636
AAACGCG6300.010.0000636
TTCTTAT4150.010.0000636
GCCTCGG5300.010.0000636
CAGAGGG4450.010.0000636
CGGAGCC2050.010.0000636
GTTTGGC11900.09.9995335
TCGTGTC1052.3646862E-119.9995335
Produced by FastQC (version 0.11.5)