FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711100
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT355838795.88496672145726No Hit
CTTTCCTA682601.8393468243916897No Hit
CTTTCCAT103450.27875831963568753No Hit
CTTTACTT82990.22362641804316777No Hit
CTTTCATT79430.2140335749508232No Hit
CATTCCTT69620.1875993640699523TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT67380.18156341785454447No Hit
ATTTCCTT62610.1687100859583412No Hit
CGTTCCTT51960.14001239524669234No Hit
CTTACCTT47970.12926086605049716No Hit
CTGTCCTT45890.1236560588504756No Hit
CTTTCCGT45350.12220096467354692No Hit
CTTTCGTT38830.10463204979655627No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)