FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166068
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG212232697.9805804803912No Hit
GAGAAGGA132360.6110611485881329No Hit
GTGAAGGG41420.19122206689725346No Hit
GAGCAGGG31430.14510163115839392No Hit
GAGAAGGC25570.11804800218645028TruSeq Adapter, Index 20 (100% over 8bp)
GAGAACGG22810.10530601994027887No Hit
GAGATGGG22360.10322852283492485No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)