FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596850
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT348948597.01502703754674No Hit
TCACTCCA229520.6381139052226253No Hit
TCCCTCCT141340.39295494668946435No Hit
ACACTCCT118830.3303724091913758No Hit
TCACACCT80260.22313969167465975No Hit
TCACTACT75580.21012830671281818No Hit
TAACTCCT51050.14192974408162698No Hit
TCAATCCT48420.13461779056674592No Hit
TCACTCAT41550.11551774469327329RNA PCR Primer, Index 45 (100% over 8bp)
TCACTCCG41510.11540653627479601No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)