FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30664733
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGG904950.29511099933594725No Hit
GAGCGATGAGGTGTCC314580.10258690333289386No Hit
CTTAGAGACAATCGCT311020.10142596056518736No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGC21950.010.00173310
CGCAAGG501.6432289E-410.00173310
TCCGCAG5350.010.0014559
CAGTCGG14200.010.0014559
CGCGTAT11600.010.0014559
TCGCGGT13100.010.000851
CTTTTCG10500.010.0005418
GGATATT6400.010.000547
CTATGGT2100.010.0005085
TCACCGG9950.010.0005086
GATGTCG5850.010.0005086
CGACGTC2550.010.0005086
GTCTCGG3300.010.0005086
TCGTGGC3300.010.0005085
AATTGGC11200.010.0005085
GGTGGCA1001.0004442E-1010.0005086
ACGGAGC4850.010.0005085
CAGGATA3000.010.0005085
GCGGAGA1900.010.0005085
GTCTTCA5600.010.0005086
Produced by FastQC (version 0.11.5)