FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4730069
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA461659197.60092294636716No Hit
GCCAAAAA148610.31418146331480573No Hit
CCCAACAA127980.2705668775656338RNA PCR Primer, Index 36 (100% over 8bp)
GCCCACAA91110.1926187546101336No Hit
GCCACCAA84380.17839063235652589No Hit
GACAACAA83610.17676274912691547No Hit
GCAAACAA78360.1656635452886628No Hit
GCCAACCA66400.140378501878091No Hit
GGCAACAA61280.1295541354682141No Hit
GCCAACAC53240.1125564975902043No Hit
TCCAACAA51020.10786311996717174No Hit
ACCAACAA47850.10116131498293154No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)