FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2831704
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA275923497.44076358263433No Hit
GTGGCGCA178410.6300446656853965No Hit
GGGGAGCA70630.24942578744106023No Hit
GTGGAGCC46920.1656952845353893No Hit
GTGGAGCT43540.1537590087099499No Hit
GAGGAGCA42560.15029819500908287No Hit
GTGGAGAA38440.13574865169523367No Hit
GTGCAGCA33980.11999841791373675No Hit
GTGGACCA33970.1199631034882177ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGGTGCA31100.10982786336424995No Hit
GTGGAGGA30840.10890968830075459No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)