FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460686
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT428441.741140478712034No Hit
GAAGCAGTGGAACCTT160130.6507534890676827No Hit
GTTCTTCCTAACGTAA126360.5135153367800687No Hit
GATACGTACCGCTCGA115990.47137261722950424No Hit
CGCTTAAGATAATAGG101860.4139496059229012No Hit
CATGGAGGATCGACAT99010.40236746988441435No Hit
TGTCCCATGAGACAAT98850.4017172447033063No Hit
AAAGCATGATCTTTGT91370.3713192174865058No Hit
TGAGCGCACAGGACAC88900.36128136625315055No Hit
TGTCGAAACTTCGCAA83050.3375075080688881No Hit
TAATTGCCTGTCTGGC80710.3279979647951831No Hit
GGGGGGGGGGGGGGGG77570.315237295615938No Hit
GGAAGGACTAGGTAAT75250.3058090304898715No Hit
CATGTCAGAGGTTTGT64770.2632192811272954No Hit
AATGATGGAAAGCTAA64440.2618781916912601No Hit
TCGACCTTGTTTCGCC60830.24720748604251008No Hit
GTGAGATCTCGATTAC53580.21774415752355236No Hit
ATGGGTACTTGGCTGC50620.20571499167305377No Hit
AAACGACCTAGGGAGG48670.19779037227829963No Hit
CAGCTCACTGTTTACG46650.1895812793668107No Hit
AGCCTACCTTAACTCG46570.1892561667762567No Hit
GCTATGATGACCTGCG46410.18860594159514865No Hit
TTGCGATGACATTCAG45630.18543609383724702No Hit
TGTTCCTCTTTATGGC44800.1820630507102491No Hit
CCTGTTGACCCTAGTA42750.17373204057730243No Hit
CAATGTGACCAACGCC42260.1717407259601591No Hit
CTGCCTTGACCATAAC40950.16641700728983708No Hit
TAACCAGACATCACAC40700.16540103044435575No Hit
AGCTTGTGACTCACTC40310.16381610656540493No Hit
CATGCAAACGAAGGCT40140.16312524231047765No Hit
CACAGCTCTGGACTGA39350.15991475547875675No Hit
CATGATGTGTCATGTG39060.15873622233799844No Hit
ATGGGTACTTTCGGGC38800.15767960641869788No Hit
ATGCCGCGATGCCACG38530.15658235142557808No Hit
CTATTCAACCGAACGA38230.1553631792110005No Hit
TGGGCCATGCGTTCTC37120.15085224201706351No Hit
TTGTTTGTGATACGCT36940.150120738688317No Hit
AGAACGCGAGGAATAT36810.14959243072866674No Hit
ATTAGCGTGGTCGGTC36360.14776367240680038No Hit
CATTAGTCTATTCTGG35590.14463446372271796No Hit
CCGCTATTGTTCGCAA35220.14313081799140565No Hit
GGGTCGTACCGCTCGA34260.13922946690475746No Hit
CACTATACTACGCCAC34060.1384166854283724No Hit
GGGCTGACTAATACAG34040.1383354072807339No Hit
AGCTCGGACCTTAAGC33200.13492172507991673No Hit
GGTAGCCTGTGCCACG33020.1341902217511702No Hit
CCTACGGGATGGAACG32840.13345871842242368No Hit
CCCAATGCTGGAATGC32790.1332555230533274No Hit
AACGTCCCTTACAGAA32300.13126420843618405No Hit
ATAGGAAACAGACTTC32260.13110165214090705No Hit
AACCAACACCGGAATG31990.13000439714778725No Hit
CTGATTACTGCTTTGA31980.12996375807396798No Hit
TTGTAACCTCATCCTC31970.12992311900014872No Hit
GGCTTGCGAGTACGTT31590.12837883419501717No Hit
ATAGCCAACATGGCCC31450.12780988716154762No Hit
CTGCCATTGCACAGTT30860.12541218180621175No Hit
TTGACGTGACCTCAAA30800.12516834736329624No Hit
CGCTTACTGGCCACGT30690.12472131755128447No Hit
TGGAGTTACAAGTCTC30540.1241117314439957No Hit
CGATCAAACCGATCGC30290.1230957545985144No Hit
CGCTGAGTGTTAGCTG30270.12301447645087589No Hit
GCAGATACTCGGCTCA29980.12183594331011759No Hit
TGTTTGTTGCGCTCGA29750.12090124461227479No Hit
CGCTTCCCTGCGCTGT28970.11773139685437313No Hit
TCAGGCTCTTAAAGCA28930.11756884055909612No Hit
TGGGTGCACTTCTGAG28670.11651222463979556No Hit
CATGTTACTAACGTGG28610.11626839019688005No Hit
ATCTTACTGCGCACTG28570.11610583390160305No Hit
GATACCATGTCTGGAT28540.11598391668014528No Hit
TCCAAAGTGAAGGCCA28440.11557752594195277No Hit
AGTGGTACTCAACGCC28280.11492730076084474No Hit
CTCGGATCTTTCTGAG28270.1148866616870255No Hit
GCGTCGATGCTTGAAC28150.11439899280119446No Hit
GTAAGAAACGACAAAT28000.11378940669390569No Hit
CGAGTCTCTTACTTTC27790.11293598614370139No Hit
AGCCGGTACGCGCAAA27720.11265151262696661No Hit
GCGACGTTGCTTAGTG27290.1109040324527388No Hit
ACCTGCTTGCCAAACT27220.11061955893600403No Hit
TTCTAACACTACGTTG27220.11061955893600403No Hit
CCTCCCTTGTTTCGCC26840.10907527413087244No Hit
GTCCTGACTTTACGAC26770.10879080061413768No Hit
ATCGCAATGTAACTCG26660.1083437708021259No Hit
CTTAGGCCTTATGACC26540.10785610191629488No Hit
TGAGATAGACTCATCC26530.10781546284247562No Hit
CCTTCAGTGTAGCTTG26430.1074090721042831No Hit
TACAACCGATTCCATT26200.10647437340644032No Hit
AACAGCTACTCGAGAG26110.10610862174206706No Hit
TTCTATGACCAACGCC26000.10566159193005527No Hit
TACGCCTTGGGTTGAG25940.10541775748713976No Hit
GAAAGTAACCCTGCGA25930.10537711841332052No Hit
TCCGGGATGCGAGTTT25700.1044424197154777No Hit
TATCTCCCTGGAATTA25660.10427986342020071No Hit
ATTGAGAGATGATCAC25530.10375155546055043No Hit
GCGACAAGAAAGGCTG25300.10281685676270763No Hit
GGTCAGAGAACCAGTT25280.10273557861506914No Hit
AGAACAATGAGTCGGA25250.10261366139361137No Hit
TGGTCCTTGTTTCTGT25180.1023291878768766No Hit
AACCACTGAAAACATG25040.1017602408434071No Hit
ACCTCCATGGAATTGA24810.1008255421455643No Hit
TTCACGCTGTGGGCAA24770.10066298585028727No Hit
GTTGCTAGACGCTCGA24750.10058170770264878No Hit
CATGTCAGAACAGGTG24630.10009403881681776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)