FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525296
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA247208797.8929598748028No Hit
GGCAAGAA81980.32463521108020604No Hit
GGCAAGGC69300.2744232755288885No Hit
GGCAGGGA39720.15728849212131962No Hit
GGCAACGA34350.13602365821670015No Hit
GGCAAGCA33850.1340436923037933Clontech Universal Primer Mix Long (100% over 8bp)
GGAAAGGA27590.10925451907419961No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)