FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20035225
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG235400.11749306533867226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17050.09.6009791
TATACTG21050.09.5099915
TACACCG10150.08.8269395
TACACTG34100.08.6621225
GAACCGT9500.08.5475116
GTCCTAG29500.08.1101351
GTGTAGG30500.08.0735931
CTAGGAC27950.08.0110193
TAGGACC21350.07.9980134
ATACCGT5450.07.96343566
TAGACAG30700.07.93427135
CCTACAC30850.07.8476663
GTCTAAG18650.07.72702651
TTATACT22950.07.6233784
TCTACAC27400.07.55893663
TAGACTG26750.07.53589635
TAGACCG8200.07.5116445
CTACACT33650.07.4869964
AGGACTG44300.07.45769265
TCTAGAC19550.07.44451053
Produced by FastQC (version 0.11.5)