FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004948619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18209623
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCTACCGTTCTC1852781.0174730141310449No Hit
AAACGACCTCTTCAAG1844321.0128271189359603No Hit
GCACTGTCTCTTTGTT1843161.0121900931172492No Hit
ATGGGTTACAATCGAA1795090.9857919628539262No Hit
TATGCTTTGGACTGTT1758480.9656872083513205No Hit
AAAGCGGACTCGGGCT1628870.8945105563140984No Hit
TCCAAAGTGCTCACGG1538460.8448609836678114No Hit
GCCTCTTGATCTGTGA1515910.832477421416138No Hit
GCAGGTCTGGCTGTTA1499620.823531601944752No Hit
TAGTGCATGAACCCGC1463710.8038112595741274No Hit
TGTGGCTCTTACGATC1382310.7591096202266241No Hit
TAACTCACTGGTGTCC1366410.7503779732287703No Hit
GAAGTATGAACAGGTG1290900.7089108873917928No Hit
TTCCCATCTCTTTGTT1248210.6854672389428381No Hit
TCACCTGACTCAATGC1202000.6600905466302076No Hit
TGAGAATGAGCAGTTT1150730.631935103763543No Hit
ATGTCCAGAGTCGGTC1090930.599095324488596No Hit
GGAACTACTACCAAGG1090340.5987713199773548No Hit
TTCGTCCGACCTGTGG1077370.5916487123319357No Hit
TCGTATACTGCTAATC1071570.588463583238379No Hit
CTAGAGACTAATCTGC1065550.5851576389033425No Hit
TCACACAGATTGGGAG1039650.570934390020046No Hit
TGCGGTGACCTTAGTG1039330.5707586587597119No Hit
ATTCCTAACTGCTACA1032610.5670683022926943No Hit
GAGCCTGACCGAGTTT1030580.5659535071099495No Hit
TCCGCGAGAGAACAGC1020930.5606541112904974No Hit
AGGTCGAGAAGGGAGG1007530.5532953647640042No Hit
GAAGTAATGAGTCTGT996550.5472655858937882No Hit
ACTGATTCTCGGCTCA996330.5471447706523084No Hit
TGGACCTCTAGGGAGG975640.5357826463513276No Hit
TGGCCTGGACCCGAGT962750.5287039715209919No Hit
CCATGATGATTCGGGC950180.5218010279509905No Hit
TTCGATGGAAATCCTG851380.46754400132281704No Hit
ACGAGGATGGAGCTGA832650.45725823099138296No Hit
GCAATCATGGAACGTG826110.45366672335830344No Hit
AAGGTGCGAACAGATA813510.4467473049826457No Hit
TTTCGTAACAGTACGG803390.44118980387457774No Hit
TCCCAGACTGTCTTGA789350.4334795948274162No Hit
TGGTCCTTGGAGAGGG774290.42520924238793956No Hit
TCTCTCTACTCTGGAT768390.42196919727552845No Hit
TCGCCGAGAAGGTAAT674490.3704030555712219No Hit
TGATATCCTCGATGTT672590.36935965121298775No Hit
TTCGATGGACAGGTGA671380.3686951673848492No Hit
TCCTTCACTTGTCACA661600.3633243807408863No Hit
AGCTGCTCTTGTCACA656070.3602875248982365No Hit
AACTCCCTGCTTCACC652130.35812383375537205No Hit
AGCCCAGTGAAAGTAC645000.3542083216110515No Hit
CTTTCGGTGCTTTGTT643960.35363719501496543No Hit
AACCCTCACGCGGTTC621410.34125363276329224No Hit
CCCTTGCCTCCTACGG612010.33609152699097616No Hit
ATGGCCGTGATACGCT606820.3332413856124314No Hit
GCGGTTCGAGTACGTT606050.33281853226725233No Hit
TCGGCTTACCTCCTAC590390.3242186837146491No Hit
GGGGGGGGGGGGGGGG582410.31983638541006587No Hit
TGTTTGTTGCAAGTTC561790.3085127023222831No Hit
AAACACGCTGCAGTTT555080.3048278374571511No Hit
ACCCTTGTGGGCCATC547030.30040709793936976No Hit
TGGCCTTTGCGAAAGC532760.29257058204884306No Hit
TACAACGTGAGCAGGT525550.2886111370894389No Hit
AAATAGGACTCTATCT520150.28564567207129987No Hit
CAATGTGTGGTTGACT519040.28503610426201575No Hit
CTAACTGGAACCTTGA518850.28493176382619234No Hit
TCAAAGGCTCTTAGTG500100.27463501029098736No Hit
ACAGCCGACAAACATG485550.26664472954766827No Hit
CTTTCGGTGAGCCATT483100.26529928708573486No Hit
TGAACTGTGTGCTGGC482260.2648379925273577No Hit
AGTGTTCGATTCGCCT457920.25147143353819024No Hit
CAGCCGATGTAATAGG445530.24466733880212677No Hit
GGACATTTGGCCGCTA424770.23326677328794782No Hit
GAGGTTGGAGGAATAT391370.21492482299056934No Hit
TTGGGAGGAATCTGTG384250.21101480244813414No Hit
CGACGTTCTTGCTACA378540.20787909777154637No Hit
TGTACTGGAAATGCTC372770.204710443483646No Hit
AGGGACCCTAATCCTG361930.19875754703982612No Hit
GTGCTAATGGGAGTCA361220.1983676433059597No Hit
TAACGAGCTTACTTTC343490.18863103316306987No Hit
GGAACATCTTTACGAC342110.1878731921028788No Hit
TCTTGAACTCGTACAT340740.18712084264457315No Hit
GGGTGAAACCGACTAT340080.18675839692013393No Hit
CGGATGAGAACAGGCA336640.18486928587154167No Hit
TACCACCTGAAACATG334070.18345794418698289No Hit
TACGTGTGAAACGCAG333640.18322180530590887No Hit
GGACCACTGGCTAGAG330470.18148096750822354No Hit
AGCTTGTGAACCGTGC327550.1798774197576743No Hit
ACTTATCTGTCAGGTT318680.17500636888528665No Hit
CATGATGTGCCTAGTA317830.174539582725024No Hit
ACGTGTTGATCTTTGT296830.16300721876559443No Hit
TCGGACCGAATGTCCA287790.15804281066115428No Hit
TGGTAGGGATGTGCTA286860.15753209168580812No Hit
AACTAGCACTGCCACG280740.1541712313319172No Hit
AACGAACCTGACACCC280050.15379231080182165No Hit
CGTCGTACTTGCGCCA277840.15257866678513882No Hit
AACTACGACATTGCCA275590.15134305636091422No Hit
GTCCGAGTGGTCTTGA274950.1509915938402459No Hit
CTCGGATCTAGTACGG270720.14866864624270365No Hit
CCTTCAGTGCAACGCC267730.1470266572789563No Hit
TACCACCTGTACTTTC266820.146526921507381No Hit
GTAGATAGAGCTGAGA265990.14607111855088928No Hit
TTTAGGGACTGGGCAA264770.14540114312086527No Hit
AGTTTGCGAGCAGGAT263420.1446597768663305No Hit
GGTAGTTTGTCCAGCG256260.14072778991635357No Hit
TGAGGAGTGGTCCCAG255530.14032690297871625No Hit
CTTTAACTGTTCGCCT251270.13798748057551768No Hit
GCCATAATGACCGTTA243180.13354477465019457No Hit
AGAACGCGACACAGTT240380.13200712612227064No Hit
CTTACATTGGAAGGCT233120.12802022315343925No Hit
CGATCGGTGCGAGGAT231620.12719648287062285No Hit
CCGTTACTGTTGGTTG228940.1257247335653242No Hit
GTCCGTTACGGATGGT227970.1251920481824363No Hit
GGCTTGCGAATACGCT225090.1236104668394288No Hit
GGGATGTGATGCATGT224120.12307778145654086No Hit
CAAAGACCTGCTTTGA223740.12286910058489403No Hit
TGCAATGACTCCGTAA222310.12208380151527574No Hit
CAGGCCATGTCGTAGT218090.11976634551961894No Hit
AATTCTGCTTAGCGAG213160.11705898579009572No Hit
AACTACGACGCTGTTA212690.11680088050147991No Hit
TCCTCATCTTTCTGGA210710.11571354332816225No Hit
CCCACCTCTTGACCTA209620.115114958722649No Hit
GAACCATACTTTGGGT205160.11266570428174157No Hit
GAAAGTCCTTATCTGG202950.11145206026505876No Hit
ATGGCCGTGGGCCAAA202660.11129280381038092No Hit
GAGAAGAGATGCTACA202050.11095781609536891No Hit
TGTCTTCCTGAAATCA200860.11030431547100124No Hit
CCTGAGGACTGACTCA200380.11004071858049999No Hit
TCGTAGAACCGCACTG197290.10834381359789821No Hit
TCCGTAAGATTACGCA193560.1062954460946281No Hit
TATGGGTACCCTTTGC191930.10540031498730093No Hit
GATAAGAACATTGGTC188440.10348374592928146No Hit
AGGTGATCTGTTAGTC188400.1034617795217397No Hit
ACAAGACCTAAGCTAA182870.1004249236790899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)