FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18209623
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG409550.22490855521830408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG32550.09.5051715
TAGACAG49400.09.4087035
GTGTATA54600.08.6411711
ACTGATC28100.08.4709198
TACACTG39100.08.3783355
GTGTTAG31200.08.3014221
ATACCGA2408.208348E-68.1655026
TATACCG2253.233497E-58.0887145
CTAGACA46500.08.0681324
ATACTGT43800.08.0216876
GTATTAG26500.07.92466161
TCTATAC22650.07.9104623
GTACTAC11550.07.878921
TACGCCG1600.0061017577.874835
CTAATAC25200.07.83210853
TTATACT30800.07.77206754
TGTACGG2901.5597925E-67.723975
GTCTAGA25600.07.71106771
TAGGACT27250.07.70576674
CTAGGAC22900.07.64072663
Produced by FastQC (version 0.11.5)