FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478082
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT241612497.49975989495101No Hit
TCACTCCA148860.6007065141508634No Hit
ACACTCCT77200.3115312568349231No Hit
GCACTCCT52890.21343119396371873No Hit
TCCCTCCT50430.20350416168633648No Hit
TCACACCT38730.15629022768415252No Hit
TCACTCAT36440.147049209832443RNA PCR Primer, Index 45 (100% over 8bp)
TCACTACT28380.11452405529760518No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)