FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30749307
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGG911380.29639041946538824No Hit
GAGCGATGAGGTGTCC308790.10042177535903492No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG553.893469E-510.00174710
CGGCAGT10750.010.0014379
GTTATGC12500.010.0008841
CTCAGTC38300.010.0005748
AGAGTTT5950.010.0005267
CCTCCGG12950.010.0005267
ATGGCGT1450.010.0005257
CCTCAGT5700.010.0005257
CACGTCG954.1472958E-1010.0005257
GCTCCGG954.1472958E-1010.0005257
AGTGGCC9600.010.0004786
AGTCTAA5400.010.0004785
TGGGATC9100.010.0004785
GCGCTCA2450.010.0004785
GTACGGC2700.010.0004786
CGTCTCC4750.010.0004775
ATACGTC7800.010.0004776
GGCGACC5750.010.0004775
TATACGT705.2604264E-710.0004775
CGGAGGC1650.010.0004776
Produced by FastQC (version 0.11.5)