FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30749307
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG395020.12846468377319853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG37100.08.75017455
GTCCTAG45400.08.1358431
TAGACAG51550.08.0074835
GTGTATA54750.07.97305971
TAGACTG42150.07.90099955
GTATTAG31650.07.86866041
TCTATAC25100.07.8579243
CTAGGAC45900.07.83218433
TACACTG59250.07.6281085
CTTAGAC31550.07.6259083
GTCTAGA36350.07.5440781
GTCCTAA37050.07.51483351
GTATAGG25350.07.3957351
CTAGTAC20450.07.3874223
CCAGGAC100450.07.3666193
CTATACT33400.07.33028034
CTACACT54300.07.3172444
TATGCCG7300.07.28389075
GTGTAGA43200.07.25468831
TAGGACC30850.07.16524364
Produced by FastQC (version 0.11.5)