FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30749307
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC33250.012.37873722
CTTATAC42950.011.0869381
ATCTCCG39600.010.7468910
CACGAGA39650.010.73370521
TCTCCGA42250.010.43730311
CCCACGA40500.010.3700619
TATACAC68800.09.7668953
CCACGAG48300.09.1012520
GACTCTA46300.09.04172126
TAGACCG10650.08.8069475
TCCGAGC53650.08.58487813
TTATACA62450.08.5183662
ACTCTAT49100.08.47021727
TAGACAG55250.08.3361175
TACACAT74050.08.280365
TACACTG55800.08.2790395
TACACCG13250.08.240985
CTAGTAC22200.08.071623
TATACTG37550.07.9409025
CTAGGAC48300.07.8546333
Produced by FastQC (version 0.11.5)