FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302809
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG323985598.09392550401795No Hit
GAGAAGGA200030.6056359904553973No Hit
GTGAAGGG51820.15689675061440125No Hit
GAGAAGGC40030.12119986351012123TruSeq Adapter, Index 20 (100% over 8bp)
GAGATGGG39880.12074570464111004No Hit
GAGAACGG37140.11244973596717218No Hit
GAGAAGCG34530.10454737164637737No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)