FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4373206
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGTGAACGTAA228020.5214023761972337No Hit
GATGAATGACTCCTAC224230.5127359653306979No Hit
CGACCAGACGCGCTGT212930.4868967983671476No Hit
TAGCTTTACCCTATCC198120.45303148308129093No Hit
CCAGGACTGTAACTCG151850.3472280976473553No Hit
ATCTGTACTGGGACTA143420.32795162176215803No Hit
GTTCTCGACCGTAGAG136990.31324844976431476No Hit
CTTCCTTTGGCTCAGC134700.30801201681329443No Hit
CGATCAAACCCGGCTA126140.2884382761754191No Hit
AGACCATTGAATCCAC120110.27464976495504667No Hit
TGCTGCTACTTTCTTG113480.2594892625684681No Hit
GGCAACAGAATAGCAA107990.24693554339768123No Hit
GGGGGGGGGGGGGGGG105670.24163051088835055No Hit
ACAGCCGACTTGCGTA104050.23792613473959381No Hit
AACTTACCTCACCTAA94150.2152882804971913No Hit
CGCGTTGGAGCGGTTC88960.20342055690950755No Hit
ATCGAAAGATTTCCGC87790.20074517413540544No Hit
GTAACTGTGTGAAAGT83930.19191869763281216No Hit
AGTGGAGTGAGTTGAA82720.18915184878096297No Hit
CCCTGAACTAGCTTTG82310.1882143214840554No Hit
CAATTCGGATAACGGG79410.18158303084739205No Hit
GGGTAGAACCAGTCTA73990.16918937731266262No Hit
AAGAACCCTTAACCCA73070.16708565752447976No Hit
TTCCTAAACTAATGCC71700.16395294436164223No Hit
CGCGATCGATATCTGG69580.15910524224104697No Hit
GTGAAGCGATCCCAGG69450.15880797748836895No Hit
TTTAGTCTGAGCAATA69320.15851071273569095No Hit
TGGAACACTCCTAGTA66460.15197088817677465No Hit
TGCAGATACTGCCACG66040.15101049436043032No Hit
CTTTAACTGTCCGTAA64170.1467344552257543No Hit
CACATCCTGAGCCTAT63700.14565972881222608No Hit
AGTCCTGCTCGAACGA63120.1443334706848934No Hit
TCCGGGAACGTTCTCC62550.14303007907699752No Hit
TGATAGAGAGACTGTT61600.14085775973050435No Hit
GCAACTTGAAGGTGCA60840.13911990425330983No Hit
AGGTGTGGATCATGTG60760.13893697209781566No Hit
CATAGGTACAAGGTGC60160.13756498093160943No Hit
TCTCAGAGATGCATGT59750.13662745363470186No Hit
TGCAGTATGACACCGC59440.135918591532162No Hit
TCAAAGCACTCTGCAA59160.13527832898793243No Hit
CTATTGGGACTTGTGA58720.13427220213271454No Hit
CTTAGATCTTGTCACA58080.13280874488876124No Hit
ATTTCTGACGGGTAGT57730.13200841670847427No Hit
TACGTTTACTGCACCG56350.1288528370262No Hit
TGAACTGTGAACGTAA56220.128555572273522No Hit
TGGCAATTGAGTGGTG55730.12743511282112024No Hit
ATGTACGTGTACTTTC55440.1267719837574539No Hit
AGAGTACCTTAATCGC54630.12491979568307553No Hit
GGTCTAAACTCCTACC54420.12443959877490335No Hit
TCTATGGGATGCTCAA54280.12411946750278857No Hit
GAGTGTTACACCTGCG53930.12331913932250162No Hit
CGAGTCTCTGCTACGC53910.12327340628362808No Hit
TACTGTTTGCTTAAGC52350.11970622925149192No Hit
TTCTTAGACGTCCCGA52280.11954616361543455No Hit
TCCATTTGAAATCTGC52230.11943183101825068No Hit
GGTCGCATGGTACTAC50840.11625338481653963No Hit
CTACAACGAATCTGTG50610.11572745486949391No Hit
GGGTGTGCTCGCAAAG50070.11449266281990832No Hit
GCATGATACTTGCGTA49900.11410393198948324No Hit
GTAATCTCTACACAAA49240.1125947417066564No Hit
CTCAATTTGGAATCTC49150.11238894303172546No Hit
CACCAGGTGCGCCACA49010.11206881175961068No Hit
GGGATGTGACTGGAAG48820.11163434789031204No Hit
TTGCGGTCTTTTGGGT48650.11124561705988696No Hit
CATTCTATGCCTAGCG48630.11119988402101343No Hit
CTTTCGTCTCGAACGA48520.11094835230720894No Hit
TCGGACCGATAGGCGA47950.10964496069931304No Hit
CTAGCTCACGAATAGT47870.10946202854381888No Hit
AGACTACTGGCCCTAG47430.10845590168860099No Hit
CATAGTCGAAGATTCC47160.1078385056638082No Hit
TGCGCAGACATCAGGG46630.10662658013365937No Hit
ACCAAGTTGAAGCTAA46390.10607778366717689No Hit
TGCTAGTGAAGGCGTA46320.10591771803111952No Hit
CGAGTCTCTTGGAACG46160.10555185372013118No Hit
TGAGGGATGTACGTTG46090.10539178808407379No Hit
AGCCAATTGTTATGGC46070.10534605504520025No Hit
TGTAAGCACGCGCTGT45820.10477439205928099No Hit
ACCTCAGCTAATCCGT45700.10449999382603975No Hit
CACATTACTGATGGAC45690.10447712730660297No Hit
ATGTCCAGACCGCTAG45270.10351673349025864No Hit
AGGCGATGACCTGCGA45190.10333380133476448No Hit
TCATCATTGGACTCGC44240.1011614819882713No Hit
TCCGGGTGAACACCTA44220.10111574894939776No Hit
AAAGCCTTGAACCAAA43740.10001815601643281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)