FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3202040
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG312037597.4495946334212No Hit
GAGAAGGA195780.611422718017264No Hit
GAGCAGGG120120.37513585089505436No Hit
GAGAAGGC47120.1471561879301945TruSeq Adapter, Index 20 (100% over 8bp)
GAGAACGG43020.13435185069518182No Hit
GTGAAGGG42440.13254050542778978No Hit
GAGAAGCG40180.12548250490312426No Hit
GAGATGGG39660.12385854018063486No Hit
GAGAAGTG37810.11808097337947059No Hit
GACAAGGG35820.11186618530686686No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)