FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004948677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828896
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA79582196.00975273134385No Hit
GGCAGGGA59130.7133584912944446No Hit
GGCAAGGC55720.6722194340423889No Hit
GGCAAGAA25580.3086032505887349No Hit
GGCAACGA23860.28785275836775664No Hit
GGCAAGCA20060.2420086476469907Clontech Universal Primer Mix Long (100% over 8bp)
CGCAAGGA15340.18506543643593407No Hit
GCCAAGGA14970.18060166776049108RNA PCR Primer, Index 1 (100% over 8bp)
GGAAAGGA14010.169019997683666No Hit
GGCCAGGA11960.1442883063737791No Hit
GGGAAGGA10320.12450295332586958No Hit
GGCAATGA9640.11629927035478516No Hit
GGCAAGGT9440.11388642242211326No Hit
GTCAAGGA8920.10761301779716635No Hit
GGCAAGTA8580.10351117631162414No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)