FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3721134
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA363331397.63993986779299No Hit
GTGGCGCA165690.445267491039022No Hit
GGGGAGCA95110.25559412802656395No Hit
GTGGAGCT66880.1797301575272484No Hit
GAGGAGCA66830.17959578988555638No Hit
GTGGAGCC58540.15731763489301917No Hit
GTGGAGAA56650.15223853803706075No Hit
GTGCAGCA49740.13366892995522334No Hit
GTGGTGCA43760.11759856000885752No Hit
GTGGACCA38050.10225377532762862ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)