FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087273
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC300735997.41150199545035Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC181200.5869257432044396No Hit
TGTAGATA140630.4555152718920549No Hit
AGTAGATC58440.18929326949706102No Hit
TGTAGAAC51950.16827148101253114Illumina DpnII expression Adapter 1 (100% over 8bp)
TGAAGATC50260.16279739433474136No Hit
TGTACATC46000.1489988089812595No Hit
TATAGATC38300.12405770399961391No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)