FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035650
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT327471.0787475499481165No Hit
TGCTGCTACCGTTCTC312851.0305865300676955No Hit
AAACGACCTCTTCAAG299970.9881573962742741No Hit
ATGGGTTACAATCGAA294790.9710935055095284No Hit
TATGCTTTGGACTGTT287800.9480671355393409No Hit
AAAGCGGACTCGGGCT274490.9042215011612011No Hit
GCAGGTCTGGCTGTTA268990.8861034704264326No Hit
TCCAAAGTGCTCACGG256710.8456508490768041No Hit
GAAGTATGAACAGGTG242270.7980827829295208No Hit
TAGTGCATGAACCCGC241370.7951180142638314No Hit
TGTGGCTCTTACGATC227660.7499547049231631No Hit
TAACTCACTGGTGTCC225690.7434651557327097No Hit
GCCTCTTGATCTGTGA206100.6789320244428705No Hit
TTCCCATCTCTTTGTT203540.6704989046826874No Hit
TCACCTGACTCAATGC197360.6501408265116203No Hit
TGAGAATGAGCAGTTT187920.6190436973959448No Hit
GGAACTACTACCAAGG187810.6186813367812495No Hit
GAAGTAATGAGTCTGT182330.6006291897946073No Hit
ATGTCCAGAGTCGGTC179800.5922948956566139No Hit
CTAGAGACTAATCTGC178580.5882759870209017No Hit
TCGTATACTGCTAATC176550.5815887865860689No Hit
TCACACAGATTGGGAG176050.5799416928829081No Hit
TTCGTCCGACCTGTGG174750.5756592492546901No Hit
TGCGGTGACCTTAGTG173330.5709815031377136No Hit
CCATGATGATTCGGGC172940.5696967700492481No Hit
ATTCCTAACTGCTACA169500.558364765371502No Hit
TCCGCGAGAGAACAGC168310.5544446823579794No Hit
AGGTCGAGAAGGGAGG166110.5471974700640719No Hit
ACTGATTCTCGGCTCA165070.5437715151614975No Hit
TGGACCTCTAGGGAGG162250.5344819066756707No Hit
GCAATCATGGAACGTG157700.5194933539769078No Hit
TGGCCTGGACCCGAGT152680.5029565331971736No Hit
TTCGATGGAAATCCTG140020.4612521206331428No Hit
ACGAGGATGGAGCTGA136290.44896480160756347No Hit
AAGGTGCGAACAGATA134250.4422446592986675No Hit
TTTCGTAACAGTACGG132460.43634806384135194No Hit
TCTCTCTACTCTGGAT128490.42327013983825534No Hit
TCCCAGACTGTCTTGA128440.42310543046793936No Hit
TGGTCCTTGGAGAGGG128390.4229407210976232No Hit
GCGGTTCGAGTACGTT125350.4129263913824057No Hit
TTCGATGGACAGGTGA114580.3774479930163227No Hit
TCGCCGAGAAGGTAAT110860.36519361586480653No Hit
TCCTTCACTTGTCACA110520.36407359214665724No Hit
TGATATCCTCGATGTT108770.3583087641855945No Hit
AGCTGCTCTTGTCACA107860.3553110536458419No Hit
CTTTCGGTGCTTTGTT107280.35340042495017543No Hit
AACTCCCTGCTTCACC106910.35218157560983643No Hit
AGCCCAGTGAAAGTAC102560.3378518603923377No Hit
TCGGCTTACCTCCTAC97430.3209526789979082No Hit
AACCCTCACGCGGTTC97390.3208209115016553No Hit
CCCTTGCCTCCTACGG97240.3203267833907071No Hit
ATGGCCGTGATACGCT96090.3165384678734373No Hit
TGTTTGTTGCAAGTTC93670.3085665343501392No Hit
AAACACGCTGCAGTTT93460.30787475499481165No Hit
GGGGGGGGGGGGGGGG91270.30066048457496747No Hit
CTAACTGGAACCTTGA88690.2921614810666579No Hit
TACAACGTGAGCAGGT87080.2868578393424802No Hit
AAATAGGACTCTATCT86640.28540839688369873No Hit
CAATGTGTGGTTGACT85860.2828389307067679No Hit
TGGCCTTTGCGAAAGC85640.2821142094773772No Hit
ACAGCCGACAAACATG83170.273977566583763No Hit
ACCCTTGTGGGCCATC83060.2736152059690676No Hit
TCAAAGGCTCTTAGTG82110.27048572793306214No Hit
GGACATTTGGCCGCTA81370.2680480292523842No Hit
CTTTCGGTGAGCCATT80660.2657091561938959No Hit
TGAACTGTGTGCTGGC79450.2617231894322468No Hit
GAGGTTGGAGGAATAT75160.24759112545912737No Hit
CAGCCGATGTAATAGG74140.24423105430467937No Hit
AGTGTTCGATTCGCCT73620.24251807685339216No Hit
GATAAGAACATTGGTC70030.23069194406469784No Hit
GGCTTGCGAATACGCT68760.22650832605866947No Hit
GTGCTAATGGGAGTCA67570.22258824304514685No Hit
CGACGTTCTTGCTACA66180.2180093225503599No Hit
GTAAAGCCTGAAATCA64890.2137598207962051No Hit
TTGGGAGGAATCTGTG62820.20694085286511948No Hit
TGTACTGGAAATGCTC61620.20298782797753362No Hit
GGAACATCTTTACGAC60830.2003854199265396No Hit
AGGGACCCTAATCCTG59520.19607003442425838No Hit
TAACGAGCTTACTTTC57540.18954754335974172No Hit
GGGTGAAACCGACTAT57390.1890534152487935No Hit
TCTTGAACTCGTACAT57100.18809810090096027No Hit
CGGATGAGAACAGGCA56190.18510039036120765No Hit
TACGTGTGAAACGCAG56190.18510039036120765No Hit
TACCACCTGAAACATG55210.18187208670301255No Hit
AGCTTGTGAACCGTGC54360.17907202740763922No Hit
ACTTATCTGTCAGGTT53400.17590960749757054No Hit
CATGATGTGCCTAGTA52660.1734719088168926No Hit
AACTAGCACTGCCACG52170.17185775698779504No Hit
ACGTGTTGATCTTTGT49430.162831683494474No Hit
TGGTAGGGATGTGCTA48360.15930690296970995No Hit
GTAGATAGAGCTGAGA46800.15416797061584833No Hit
CGTCGTACTTGCGCCA46470.15308088877176224No Hit
AACTACGACATTGCCA46460.153047946897699No Hit
AACGAACCTGACACCC45800.1508737832095268No Hit
TCGGACCGAATGTCCA45590.15018200385419925No Hit
CTCGGATCTAGTACGG44990.14820549141040634No Hit
GGTAGTTTGTCCAGCG44730.14734900268476275No Hit
CCTTCAGTGCAACGCC44270.1458336764778548No Hit
AGTTTGCGAGCAGGAT43670.1438571640340619No Hit
TTTAGGGACTGGGCAA43570.14352774529342974No Hit
TACCACCTGTACTTTC43280.1425724309455965No Hit
TGAGGAGTGGTCCCAG41650.1372029054732924No Hit
CTTTAACTGTTCGCCT41440.13651112611796484No Hit
AGAACGCGACACAGTT40060.13196514749724111No Hit
GCCATAATGACCGTTA40010.13180043812692505No Hit
GGGATGTGATGCATGT39670.1306804144087757No Hit
CTTACATTGGAAGGCT39650.1306145306606493No Hit
CGATCGGTGCGAGGAT39320.12952744881656317No Hit
GAAAGTCCTTATCTGG38870.12804506448371847No Hit
CAGGCCATGTCGTAGT38580.12708975013588522No Hit
GAGAAGAGATGCTACA38140.12564030767710377No Hit
CAAAGACCTGCTTTGA37960.12504735394396588No Hit
GGACCACTGGCTAGAG37600.12386144647769011No Hit
AATTCTGCTTAGCGAG36960.12175316653764431No Hit
GAAGGACACCCAGCTG36620.120633142819495No Hit
AACTACGACGCTGTTA36130.11901899099039744No Hit
TGCAATGACTCCGTAA36100.1189201653682078No Hit
GCTGGACACATTGGTC35380.1165483504356563No Hit
CCGTTACTGTTGGTTG35030.11539538484344374No Hit
TCCTCATCTTTCTGGA34640.11411065175497834No Hit
TGTCTTCCTGAAATCA34490.1136165236440301No Hit
GGCTTAAACTGCATCA34170.1125623836740072No Hit
CCCACCTCTTGACCTA34000.11200237181493256No Hit
GCGTTTAGAAGTCTAC33780.11127765058554182No Hit
ACAAGACCTAAGCTAA33460.11022351061551892No Hit
ATAGCCAACATCTGTG32950.10854347503829492No Hit
ATGGCCGTGGGCCAAA32720.10778581193484098No Hit
TCGTAGAACCGCACTG32680.10765404443858811No Hit
CCTGAGGACTGACTCA31810.10478810139508837No Hit
TATGGGTACCCTTTGC31700.104425740780393No Hit
AGGTGATCTGTTAGTC31530.10386572892131833No Hit
TCCGTAAGATTACGCA30900.10179039085533577No Hit
GCGTTAATGGTACGTT30780.10139508836657717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)