FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047511
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA295971897.11919005378488No Hit
GGCAAGGC129660.42546195895601363No Hit
GGCAAGAA94630.3105156962517937No Hit
GGCAACGA82810.271729946175748No Hit
GGCAAGCA73440.24098354361969487Clontech Universal Primer Mix Long (100% over 8bp)
GCCAAGGA57580.18894107355149825RNA PCR Primer, Index 1 (100% over 8bp)
CGCAAGGA55130.1809017260315057No Hit
GGCAGGGA44790.14697239813080248No Hit
GGAAAGGA44470.14592236090370142No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)