FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3824223
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGTGAACGTAA202670.5299638645549698No Hit
CGACCAGACGCGCTGT188530.49298903332781585No Hit
TAGCTTTACCCTATCC177720.4647218533019649No Hit
GATGAATGACTCCTAC174150.4553866236357032No Hit
CCAGGACTGTAACTCG135120.3533266757717842No Hit
ATCTGTACTGGGACTA125510.32819738807072707No Hit
GTTCTCGACCGTAGAG120440.31493979299847313No Hit
CTTCCTTTGGCTCAGC119900.31352774145231593No Hit
CGATCAAACCCGGCTA113320.2963216318713631No Hit
AGACCATTGAATCCAC104470.27317967597600873No Hit
TGCTGCTACTTTCTTG98230.25686263588708086No Hit
GGGGGGGGGGGGGGGG97110.25393393638393996No Hit
ACAGCCGACTTGCGTA93380.2441803210743725No Hit
GGCAACAGAATAGCAA92070.24075478861980587No Hit
AACTTACCTCACCTAA85210.22281650416306786No Hit
CGCGTTGGAGCGGTTC78730.20587188560918127No Hit
ATCGAAAGATTTCCGC76350.19964839916500685No Hit
GTAACTGTGTGAAAGT74800.1955952882454815No Hit
CCCTGAACTAGCTTTG74700.19533379721841534No Hit
AGTGGAGTGAGTTGAA73290.1916467737367826No Hit
CAATTCGGATAACGGG69790.18249458778946728No Hit
GGGTAGAACCAGTCTA64700.16918469451180018No Hit
AAGAACCCTTAACCCA63860.1669881698844445No Hit
TTCCTAAACTAATGCC62900.16447785602460946No Hit
TTTAGTCTGAGCAATA61570.16100002536462962No Hit
CGCGATCGATATCTGG61390.16052934151591056No Hit
GTGAAGCGATCCCAGG60880.1591957372778732No Hit
TGGAACACTCCTAGTA59090.15451504789338907No Hit
TGCAGATACTGCCACG57110.14933752555747926No Hit
TGATAGAGAGACTGTT56190.14693180810847067No Hit
CTTTAACTGTCCGTAA56000.14643497515704498No Hit
CACATCCTGAGCCTAT55990.14640882605433836No Hit
AGTCCTGCTCGAACGA55290.14457838886487528No Hit
AGGTGTGGATCATGTG53690.14039453243181688No Hit
GCAACTTGAAGGTGCA53410.13966235755603165No Hit
TGCAGTATGACACCGC52960.13848564793423396No Hit
CATAGGTACAAGGTGC52960.13848564793423396No Hit
TCTCAGAGATGCATGT52360.13691670177183704No Hit
CTTAGATCTTGTCACA51900.13571384304733275No Hit
TCCGGGAACGTTCTCC51600.1349293699661343No Hit
TACGTTTACTGCACCG51510.13469402804177477No Hit
TGGCAATTGAGTGGTG50360.131686881230514No Hit
TCAAAGCACTCTGCAA50290.13150383751156772No Hit
CTATTGGGACTTGTGA50070.13092855725202218No Hit
ATTTCTGACGGGTAGT49980.13069321532766265No Hit
AGAGTACCTTAATCGC49160.1285489889057202No Hit
TGAACTGTGAACGTAA48940.12797370864617466No Hit
ATGTACGTGTACTTTC48430.12664010440813728No Hit
TCTATGGGATGCTCAA48180.12598637684047193No Hit
CGAGTCTCTGCTACGC47330.12376370311040963No Hit
GGTCTAAACTCCTACC46300.12107034553162825No Hit
TTCTTAGACGTCCCGA46120.12059966168290918No Hit
TACTGTTTGCTTAAGC45720.11955369757464457No Hit
GCATGATACTTGCGTA45010.11769711128247491No Hit
TCCATTTGAAATCTGC44920.11746176935811536No Hit
CTACAACGAATCTGTG44400.11610201601737137No Hit
GGGTGTGCTCGCAAAG44160.1154744375524126No Hit
CACCAGGTGCGCCACA44030.11513449921722661No Hit
CATTCTATGCCTAGCG43870.11471611357392077No Hit
TTGCGGTCTTTTGGGT43630.114088535108962No Hit
GGTCGCATGGTACTAC43340.11333021113047016No Hit
AGACTACTGGCCCTAG42580.1113428793247674No Hit
CTCAATTTGGAATCTC42460.11102909009228802No Hit
GAGTGTTACACCTGCG42330.11068915175710203No Hit
CTTTCGTCTCGAACGA41910.1095908894434242No Hit
AGCCAATTGTTATGGC41860.10946014392989112No Hit
TCGGACCGATAGGCGA41840.10940784572447788No Hit
CTAGCTCACGAATAGT41690.10901560918387868No Hit
GGGATGTGACTGGAAG41660.10893716187575882No Hit
CGAGTCTCTTGGAACG41660.10893716187575882No Hit
CACATTACTGATGGAC41590.10875411815681252No Hit
NNNNNNNNNNNNNNNN41570.10870181995139927No Hit
ACCAAGTTGAAGCTAA41460.1084141798216265No Hit
TGTAAGCACGCGCTGT41450.10838803071891989No Hit
CATAGTCGAAGATTCC41130.10755125943230821No Hit
TGCTAGTGAAGGCGTA40950.10708057558358915No Hit
ATGTCCAGACCGCTAG40610.10619150609156422No Hit
GTAATCTCTACACAAA40410.1056685240374319No Hit
ACCTCAGCTAATCCGT40320.10543318211307239No Hit
TAGTACCGAACGAGCA40130.1049363491616467No Hit
TGCGCAGACATCAGGG39880.1042826215939813No Hit
TCATCATTGGACTCGC39620.10360274492360931No Hit
TCCGGGTGAACACCTA39580.10349814851278287No Hit
AGGCGATGACCTGCGA38560.1008309400367081No Hit
CTAACAGTGCATGATC38500.10067404542046841No Hit
ATAGCCAACCGTTCTC38400.10041255439340227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)