FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3660063
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT355383997.09775487471117No Hit
TCACTCCA229720.6276394695938294No Hit
TCCCTCCT148410.4054848236218884No Hit
ACACTCCT115650.31597816758891856No Hit
TCACACCT76250.20832974732948586No Hit
TCACTACT71610.1956523699182227No Hit
TAACTCCT50820.13885006897422258No Hit
TCAATCCT44960.12283941560568767No Hit
TCACTCAT43020.11753896039494402RNA PCR Primer, Index 45 (100% over 8bp)
TCACTCCG42040.1148614108554962No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)