FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327190
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCAA320225396.24496947874933No Hit
TTAGTCAA700782.106221766716058No Hit
TCAGGCAA93690.2815889684688882No Hit
TCAGACAA82280.2472957660969166No Hit
TCAGTAAA67020.20143123777121236No Hit
ACAGTCAA60740.18255645154018857TruSeq Adapter, Index 13 (100% over 8bp)
TCAGTCAT38610.1160438688502911No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)