FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357390
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA326532097.25769124230429No Hit
GGCAAGGC139150.41445885047611386No Hit
GGCAAGAA105180.31327906498798175No Hit
GGCAACGA85200.25376855235763496No Hit
GGCAAGCA70910.2112057282591537Clontech Universal Primer Mix Long (100% over 8bp)
GCCAAGGA59280.1765657251615094RNA PCR Primer, Index 1 (100% over 8bp)
CGCAAGGA52650.15681824274213005No Hit
GGAAAGGA48700.14505315140630073No Hit
GGCAGGGA47630.14186615198115204No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)