FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7655412
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA744698697.27740322793862No Hit
GTGGCGCA620610.8106813846204489No Hit
GTGGAGCC148440.19390203949833137No Hit
GAGGAGCA122020.15939050700341145No Hit
GGGGAGCA121760.1590508779932419No Hit
GTGGACCA100980.1319066824881535ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGGAGCT100150.13082248218645842No Hit
GTGCAGCA91070.11896159213899918No Hit
GTGGAGAA89520.11693688073221925No Hit
GTGTAGCA82730.1080673385050994No Hit
CTGGAGCA79450.1037827879152683No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)