FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31309798
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG396280.1265674087070124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG41800.08.2691085
TACACTG59150.08.0438175
TATACCG5450.07.9598195
TAGACAG55200.07.83352765
GTGTATA58500.07.63002971
GTATAGG28350.07.60079571
TATACTG37000.07.56425675
GTCTAGA38750.07.5107451
ACCGTCC23000.07.42482338
CTAAGAC40350.07.4201313
CCTACAC48100.07.3880533
ATACCGT8350.07.37525756
TCTATAC27400.07.3528093
TATACAG43500.07.3025515
CTAGGAC46150.07.2758023
CTATACT36650.07.21589764
TACACAG74300.07.15702965
CTAGTAC20450.07.11510663
GACCGTA6100.07.11336147
GTATAGA28350.07.1072381
Produced by FastQC (version 0.11.5)