FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4343868
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT422092597.16973443944428No Hit
CTAGCTGA247180.5690320239933626No Hit
CTAGCAGT87070.2004434757225588No Hit
CTAGCTAT73750.16977956052071563RNA PCR Primer, Index 38 (100% over 8bp)
CAAGCTGT72600.16713215042446042No Hit
CTAGCTGG71810.16531349479311988No Hit
CTAGCGGT69590.16020284225947934No Hit
CTAGATGT65060.14977434857596963No Hit
CTAGCTCT64240.14788663007255287No Hit
CTCGCTGT60890.14017460935737458No Hit
CTACCTGT58110.13377478321164454No Hit
ATAGCTGT49670.11434509520086705No Hit
CTTGCTGT49040.11289477488726637No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)