FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004948859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14093341
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1543641.0952974174115278No Hit
TGCTGCTACCGTTCTC1443341.0241290549912898No Hit
AAACGACCTCTTCAAG1409320.9999899952750736No Hit
ATGGGTTACAATCGAA1375520.9760070376499086No Hit
TATGCTTTGGACTGTT1356970.9628447931544407No Hit
AAAGCGGACTCGGGCT1258780.8931735916983773No Hit
GCCTCTTGATCTGTGA1245420.8836939374418031No Hit
TCCAAAGTGCTCACGG1198960.8507280140315913No Hit
TAGTGCATGAACCCGC1123970.797518487631854No Hit
TGTGGCTCTTACGATC1067380.7573647724836857No Hit
TAACTCACTGGTGTCC1057360.7502550317912552No Hit
GCAGGTCTGGCTGTTA1008180.7153591188916808No Hit
TTCCCATCTCTTTGTT973510.6907588484519036No Hit
GAAGTATGAACAGGTG964080.6840677451854744No Hit
TCACCTGACTCAATGC912290.6473198938420635No Hit
TGAGAATGAGCAGTTT887910.6300209439337343No Hit
GAGCCTGACCGAGTTT876550.6219603995958091No Hit
GGAACTACTACCAAGG859580.6099192519360739No Hit
CTAGAGACTAATCTGC842830.5980342063673901No Hit
ATGTCCAGAGTCGGTC829290.5884268322181376No Hit
TTCGTCCGACCTGTGG822720.5837650561353763No Hit
TCGTATACTGCTAATC813530.5772442460591849No Hit
ATTCCTAACTGCTACA802990.5697655367879058No Hit
TCACACAGATTGGGAG801270.5685451022578677No Hit
TCCGCGAGAGAACAGC777650.5517854141186253No Hit
AGGTCGAGAAGGGAGG775840.550501119642248No Hit
ACTGATTCTCGGCTCA773510.5488478565870222No Hit
TGGACCTCTAGGGAGG756300.5366364157370492No Hit
TGGCCTGGACCCGAGT749940.5321236461957459No Hit
CCATGATGATTCGGGC742530.5268658439471521No Hit
GCAATCATGGAACGTG695730.49365867185076984No Hit
TTCGATGGAAATCCTG656680.4659505506891517No Hit
ACGAGGATGGAGCTGA627270.44508253933542086No Hit
TTTCGTAACAGTACGG621580.4410451716168651No Hit
AAGGTGCGAACAGATA615830.4369652306007497No Hit
TCCCAGACTGTCTTGA608020.4314236063684261No Hit
TGGTCCTTGGAGAGGG596860.42350497302236567No Hit
TCTCTCTACTCTGGAT587440.41682096530552976No Hit
TGCGGTGACCTTAGTG539470.38278361390673793No Hit
TTCGATGGACAGGTGA520950.3696426560600499No Hit
TGATATCCTCGATGTT513540.3643848538114561No Hit
AGCTGCTCTTGTCACA513280.3642003695220317No Hit
TCCTTCACTTGTCACA513250.364179082873252No Hit
AGCCCAGTGAAAGTAC507720.36025524394818803No Hit
CTTTCGGTGCTTTGTT499010.35407502025247245No Hit
TCGCCGAGAAGGTAAT496650.35240047054846685No Hit
AACTCCCTGCTTCACC494010.3505272454558504No Hit
AACCCTCACGCGGTTC476460.33807455591970703No Hit
CCCTTGCCTCCTACGG472030.3349312274498999No Hit
ATGGCCGTGATACGCT470050.3335263086304376No Hit
GGGGGGGGGGGGGGGG451410.32030020418863064No Hit
TCGGCTTACCTCCTAC448000.31788062177733445No Hit
AAACACGCTGCAGTTT435280.30885508269472794No Hit
TGTTTGTTGCAAGTTC434230.30811004998743735No Hit
ACCCTTGTGGGCCATC421100.29879359337150785No Hit
TGGCCTTTGCGAAAGC415890.2950968120334277No Hit
GAAGTAATGAGTCTGT415180.2945930280123074No Hit
CTAACTGGAACCTTGA412610.29276947176684365No Hit
TACAACGTGAGCAGGT404610.28709303209224835No Hit
CAATGTGTGGTTGACT402790.28580164206627795No Hit
AAATAGGACTCTATCT395800.2808418529006004No Hit
TCAAAGGCTCTTAGTG390100.27679738963245126No Hit
ACAGCCGACAAACATG389950.27669095638855257No Hit
CTTTCGGTGAGCCATT375490.2664307916767217No Hit
GGACATTTGGCCGCTA370610.2629681634752185No Hit
TGAACTGTGTGCTGGC369970.2625140483012509No Hit
AGTGTTCGATTCGCCT352210.24991235222364946No Hit
CAGCCGATGTAATAGG346940.2461729975880098No Hit
GGCTTGCGAATACGCT312900.22201974677260702No Hit
GTAAAGCCTGAAATCA294120.20869430463649466No Hit
TTGGGAGGAATCTGTG293400.20818342506578108No Hit
GTGCTAATGGGAGTCA289220.20521748533580503No Hit
GATAAGAACATTGGTC287440.2039544775082076No Hit
TGTACTGGAAATGCTC283510.2011659265180627No Hit
AGGGACCCTAATCCTG277730.19706469885316763No Hit
GGAACATCTTTACGAC275420.19542562689712825No Hit
CGACGTTCTTGCTACA272380.19326857982078202No Hit
GGACCACTGGCTAGAG267210.18960018068107484No Hit
TCTTGAACTCGTACAT263860.1872231715673381No Hit
TAACGAGCTTACTTTC261300.1854067108714676No Hit
GGGTGAAACCGACTAT259370.18403726979997148No Hit
TACGTGTGAAACGCAG258050.18310065725366328No Hit
TACCACCTGAAACATG257550.18274587977400106No Hit
AGCTTGTGAACCGTGC254060.18026953296595888No Hit
CGGATGAGAACAGGCA252460.17913424503103983No Hit
CATGATGTGCCTAGTA251400.17838211677415597No Hit
ACTTATCTGTCAGGTT246280.17474919538241498No Hit
AACTAGCACTGCCACG236350.16770331463632362No Hit
AACTACGACATTGCCA220340.1563433397375399No Hit
ACGTGTTGATCTTTGT220050.1561375687993358No Hit
TCGGACCGAATGTCCA218900.15532158059611273No Hit
CGTCGTACTTGCGCCA217180.15410114606607475No Hit
TGGTAGGGATGTGCTA216230.15342706885471655No Hit
AACGAACCTGACACCC214070.15189443014257584No Hit
CCTTCAGTGCAACGCC209890.1489284904125998No Hit
GTAGATAGAGCTGAGA208900.14822603100286866No Hit
GTCCGAGTGGTCTTGA208440.14789963572157944No Hit
CTCGGATCTAGTACGG207280.14707655196876313No Hit
TTTAGGGACTGGGCAA206780.14672177448910093No Hit
TACCACCTGTACTTTC202960.14401127454448168No Hit
AGTTTGCGAGCAGGAT202850.143933223498956No Hit
GGTAGTTTGTCCAGCG197560.14017967776412987No Hit
TGAGGAGTGGTCCCAG197400.14006614897063796No Hit
CTTTAACTGTTCGCCT194360.13790910189429179No Hit
GCCATAATGACCGTTA193840.1375401333154431No Hit
AGAACGCGACACAGTT185670.13174306929776267No Hit
CTTACATTGGAAGGCT181730.1289474227580245No Hit
CAGGCCATGTCGTAGT176270.12507325268011324No Hit
CAAAGACCTGCTTTGA174650.12392377364600772No Hit
CCGTTACTGTTGGTTG173310.12297297000051302No Hit
GTCCGTTACGGATGGT173100.12282396345905489No Hit
TGCAATGACTCCGTAA171900.1219724975078656No Hit
GGGATGTGATGCATGT170910.12127003809813443No Hit
GAAAGTCCTTATCTGG168530.11958129729494234No Hit
CGATCGGTGCGAGGAT167510.11885755123643145No Hit
GAACCATACTTTGGGT166330.11802027638442864No Hit
AACTACGACGCTGTTA165520.11744553686737588No Hit
AATTCTGCTTAGCGAG164860.11697723059422177No Hit
TCCTCATCTTTCTGGA164220.11652311542025415No Hit
GAGAAGAGATGCTACA163060.11570003166743784No Hit
CCCACCTCTTGACCTA159090.11288309847891993No Hit
TGTCTTCCTGAAATCA156340.11093182234077781No Hit
GCTGGACACATTGGTC156010.11069766920420077No Hit
ATGGCCGTGGGCCAAA155870.11059833150989534No Hit
TCGTAGAACCGCACTG154330.10950561687253575No Hit
ATAGCCAACATCTGTG154050.10930694148392492No Hit
ACAAGACCTAAGCTAA152970.10854062212785458No Hit
CCTGAGGACTGACTCA150710.1069370279197814No Hit
GGCTTAAACTGCATCA150310.10665320593605165No Hit
TCCGTAAGATTACGCA149960.10640486170028811No Hit
TATGGGTACCCTTTGC147040.10433296121906083No Hit
GAGGTTGGAGGAATAT146990.1042974834710946No Hit
AGGTGATCTGTTAGTC145010.10289256465163228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)