FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696926
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT674041.8232445009718885No Hit
GAAGCAGTGGAACCTT223210.6037718904841483No Hit
GTTCTTCCTAACGTAA188890.5109380063328289No Hit
CGCTTAAGATAATAGG157970.42730095219650055No Hit
CATGGAGGATCGACAT153930.4163729541786879No Hit
TGTCCCATGAGACAAT152090.4113958461705753No Hit
AAAGCATGATCTTTGT144380.39054068163658134No Hit
TGAGCGCACAGGACAC134000.3624633005908152No Hit
GATACGTACCGCTCGA131420.35548452957943977No Hit
TGTCGAAACTTCGCAA129460.350182827570798No Hit
TAATTGCCTGTCTGGC124920.3379023545507808No Hit
GGAAGGACTAGGTAAT108240.29278378847723757No Hit
GGGGGGGGGGGGGGGG106980.28937555147168215No Hit
AATGATGGAAAGCTAA101660.2749852174482259No Hit
CATGTCAGAGGTTTGT99830.27003515894015734No Hit
TCGACCTTGTTTCGCC94440.25545547841639243No Hit
GTGAGATCTCGATTAC80220.21699108935369546No Hit
ATGGGTACTTGGCTGC79360.21466483234990366No Hit
AAACGACCTAGGGAGG72950.19732610282164154No Hit
AGCCTACCTTAACTCG71770.19413426181643884No Hit
TTGCGATGACATTCAG70470.19061782681070705No Hit
GCTATGATGACCTGCG69390.18769648080594525No Hit
TGTTCCTCTTTATGGC68550.18542432280224164No Hit
CTGCCTTGACCATAAC65880.17820210629046945No Hit
CAGCTCACTGTTTACG65210.17638978978751535No Hit
CCTGTTGACCCTAGTA65070.1760110967868981No Hit
CAATGTGACCAACGCC63840.17268400828147493No Hit
CACAGCTCTGGACTGA62830.16995200877702177No Hit
CATGCAAACGAAGGCT62580.1692757712759195No Hit
CATGATGTGTCATGTG61400.1660839302707168No Hit
TAACCAGACATCACAC61100.16527244526939408No Hit
ATGGGTACTTTCGGGC60990.16497490076890908No Hit
ATGCCGCGATGCCACG58700.15878056525881232No Hit
CTATTCAACCGAACGA57390.15523708075303644No Hit
AGAACGCGAGGAATAT57220.1547772392522869No Hit
TGGGCCATGCGTTCTC56640.15320836824972964No Hit
TTGTTTGTGATACGCT56280.15223458624814237No Hit
CATTAGTCTATTCTGG55860.15109850724629056No Hit
ATTAGCGTGGTCGGTC55030.14885339874263104No Hit
AGCTTGTGACTCACTC54720.14801486424126423No Hit
CCGCTATTGTTCGCAA53620.14503941923641425No Hit
CACTATACTACGCCAC52670.14246971673222564No Hit
GGGCTGACTAATACAG52210.14122543973019747No Hit
CCCAATGCTGGAATGC50820.13746555922406886No Hit
AACCAACACCGGAATG50270.13597783672164387No Hit
AACGTCCCTTACAGAA50060.13540979722071797No Hit
ATAGGAAACAGACTTC50040.1353556982206298No Hit
CTGCCATTGCACAGTT49940.13508520322018888No Hit
AGCTCGGACCTTAAGC49010.13256959971608845No Hit
ATAGCCAACATGGCCC48870.13219090671547118No Hit
CCTACGGGATGGAACG48710.13175811471476573No Hit
GGTAGCCTGTGCCACG48250.13051383771273756No Hit
TTGACGTGACCTCAAA48180.13032449121242892No Hit
GCAGATACTCGGCTCA48180.13032449121242892No Hit
CTGATTACTGCTTTGA48080.13005399621198802No Hit
TGGAGTTACAAGTCTC47120.1274572442077553No Hit
TTGTAACCTCATCCTC47060.12729494720749077No Hit
CGATCAAACCGATCGC46950.12699740270700577No Hit
CGCTTACTGGCCACGT46880.12680805620669713No Hit
TCAGGCTCTTAAAGCA46870.12678100670665304No Hit
ATCTTACTGCGCACTG46180.12491459120361077No Hit
CGCTGAGTGTTAGCTG45940.12426540320255261No Hit
TGTTTGTTGCGCTCGA45930.1242383537025085No Hit
GCATCTCTGAGCCTAT45890.12413015570233216No Hit
TCCAAAGTGAAGGCCA45290.1225071856996867No Hit
GGGATCAGATGAGTAA44810.12120880969757036No Hit
TGGGTGCACTTCTGAG44800.12118176019752626No Hit
CTCGGATCTTTCTGAG44130.1193694436945722No Hit
TTCTAACACTACGTTG43800.11847681019311719No Hit
CGCTTCCCTGCGCTGT43680.1181522161925881No Hit
CATGTTACTAACGTGG43520.11771942419188267No Hit
CTTAGGCCTTATGACC43130.11666449369016313No Hit
GATACCATGTCTGGAT42700.11550136518826723No Hit
AGCCGGTACGCGCAAA42160.11404069218588632No Hit
CGAGTCTCTTACTTTC41780.11301281118421089No Hit
TACAACCGATTCCATT41680.11274231618376997No Hit
AGTGGTACTCAACGCC41560.11241772218324089No Hit
ACCTGCTTGCCAAACT41530.11233657368310862No Hit
ATTGAGAGATGATCAC41330.1117955836822268No Hit
CCTCCCTTGTTTCGCC41240.11155213818182998No Hit
AACAGCTACTCGAGAG40610.10984801967905226No Hit
CCTTCAGTGTAGCTTG40560.10971277217883182No Hit
TACGCCTTGGGTTGAG40420.10933407917821454No Hit
TTCACGCTGTGGGCAA40370.10919883167799409No Hit
AACCACTGAAAACATG40130.10854964367693593No Hit
TGGTCCTTGTTTCTGT39900.10792750517592184No Hit
TATCTCCCTGGAATTA39900.10792750517592184No Hit
GTGTACTGAATCACAC39870.10784635667578955No Hit
ATCGCAATGTAACTCG39690.10735946567499594No Hit
TGAGATAGACTCATCC39600.1071160201745991No Hit
AGAACAATGAGTCGGA39530.10692667367429048No Hit
AGGTTACTGGTCCCGA39290.1062774856732323No Hit
GCATGGGCTATGTGGG39100.10576354517239457No Hit
ACCTCCATGGAATTGA38950.10535780267173322No Hit
TCCGGGATGCGAGTTT38740.1047897631708073No Hit
GTTGCTAGACGCTCGA38650.1045463176704105No Hit
CATGTCAGAACAGGTG38430.1039512286694405No Hit
GTCCTGACTTTACGAC38430.1039512286694405No Hit
ATTCCTAACATTGCCA38410.10389712966935231No Hit
TTCTATGACCAACGCC38040.10289629816772096No Hit
AGAGAATTGCCCGCAA38010.1028151496675887No Hit
AGAACATGAAAGCTAA37930.10259875366723596No Hit
TTCGACCACAGTACGG37830.10232825866679507No Hit
CCTTCTTTGGATCATC37350.1010298826646787No Hit
CGGTTCACTGGAATGC37310.10092168466450235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)