FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146169
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG306546997.43497567994599No Hit
GAGAAGGA191490.6086449901451575No Hit
GAGCAGGG114480.3638711080046876No Hit
GAGAAGGC47830.15202616261237079TruSeq Adapter, Index 20 (100% over 8bp)
GAGAACGG45660.14512888532052792No Hit
GAGAAGCG42020.1335592588955012No Hit
GAGATGGG37170.11814368522479245No Hit
GAGAAGTG36880.117221929273348No Hit
GACAAGGG36650.11649088144978861No Hit
GTGAAGGG36440.11582340300219092No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)