FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534145
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG243316196.01506622549223No Hit
GGACAGGA293221.157076647153182No Hit
GGCCAGGG285921.1282700871497093ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGC47300.186650724406062No Hit
GGACACGG43700.17244474960982897No Hit
GGAAAGGG36960.14584800790799263No Hit
GGACAGCG32220.1271434744262858No Hit
CGACAGGG31620.12477581196024695No Hit
GCACAGGG27000.10654481097174787No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)