FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4674690
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA457270197.81827244159506No Hit
GCCAAAAA144770.30968898472412076No Hit
CCCAACAA134610.28795492321415966RNA PCR Primer, Index 36 (100% over 8bp)
GCAAACAA78390.16769026395333167No Hit
GACAACAA76650.16396809200182258No Hit
GCCCACAA76400.16343329718120345No Hit
GCCACCAA63490.13581649264443205No Hit
GCCAACCA59020.12625436125176215No Hit
ACCAACAA53570.1145958341622653No Hit
TCCAACAA53270.11395408037752236No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)