FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3310241
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT323519397.73285389190697No Hit
CTAGCTGA184620.5577237427728071No Hit
CTAGCTAT58570.17693575784965507RNA PCR Primer, Index 38 (100% over 8bp)
CTAGCAGT51600.15587988910777192No Hit
CAAGCTGT44260.1337062769749997No Hit
CTAGATGT41240.12458307416287817No Hit
CTAGCTGG40280.12168298320273357No Hit
CTAGCGGT39390.11899435720843286No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)