FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192448
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT379121.729208628893365No Hit
GAAGCAGTGGAACCTT135600.6184867326385848No Hit
GATACGTACCGCTCGA114970.5243910003794845No Hit
GTTCTTCCTAACGTAA113490.5176405552149926No Hit
CGCTTAAGATAATAGG88810.405072320985492No Hit
TGTCCCATGAGACAAT87260.39800259800916604No Hit
CATGGAGGATCGACAT86880.39626937560206676No Hit
TGAGCGCACAGGACAC80720.3681729281606679No Hit
AAAGCATGATCTTTGT79750.3637486499109671No Hit
TGTCGAAACTTCGCAA73040.33314359109087194No Hit
TAATTGCCTGTCTGGC72790.3320033131914645No Hit
GGAAGGACTAGGTAAT64850.29578808710628485No Hit
GGGGGGGGGGGGGGGG59860.2730281402341127No Hit
AATGATGGAAAGCTAA57140.26062191668856No Hit
CATGTCAGAGGTTTGT56940.25970969436903407No Hit
TCGACCTTGTTTCGCC54670.2493559710424147No Hit
GCTATGATGACCTGCG45640.20816913331581866No Hit
ATGGGTACTTGGCTGC44920.20488513296552532No Hit
AAACGACCTAGGGAGG42280.19284379834778295No Hit
AGCCTACCTTAACTCG40460.18454257524009693No Hit
CAGCTCACTGTTTACG39850.18176029716554282No Hit
TGTTCCTCTTTATGGC39700.18107613042589837No Hit
TTGCGATGACATTCAG39700.18107613042589837No Hit
GTGAGATCTCGATTAC39700.18107613042589837No Hit
CTGCCTTGACCATAAC37490.17099607379513676No Hit
AGCTTGTGACTCACTC36330.16570518434188633No Hit
CAATGTGACCAACGCC35780.16319657296319No Hit
CACAGCTCTGGACTGA35670.16269485068745074No Hit
CCTGTTGACCCTAGTA35450.16169140613597222No Hit
TAACCAGACATCACAC35370.16132651720816182No Hit
ATGGGTACTTTCGGGC35060.15991257261289663No Hit
CATGATGTGTCATGTG34420.15699346119041363No Hit
CTATTCAACCGAACGA34230.156126849986864No Hit
CATGCAAACGAAGGCT34190.15594440552295882No Hit
ATGCCGCGATGCCACG33650.15348140526023876No Hit
ATTAGCGTGGTCGGTC33130.15110962722947135No Hit
AGAACGCGAGGAATAT32640.1488746825466328No Hit
TGGGCCATGCGTTCTC32330.1474607379513676No Hit
CATTAGTCTATTCTGG31280.14267157077385645No Hit
GCAGATACTCGGCTCA31180.14221545961409346No Hit
GGGCTGACTAATACAG31080.1417593484543305No Hit
TTGTTTGTGATACGCT30890.14089273725078086No Hit
CACTATACTACGCCAC30870.14080151501882826No Hit
CCGCTATTGTTCGCAA30630.13970684823539714No Hit
GCATCTCTGAGCCTAT30630.13970684823539714No Hit
GGGTCGTACCGCTCGA30470.1389770703797764No Hit
GGCTTGCGAGTACGTT30440.1388402370318475No Hit
AACCAACACCGGAATG29890.13633162565315118No Hit
GGTAGCCTGTGCCACG29240.13336690311469188No Hit
CCCAATGCTGGAATGC29040.13245468079516595No Hit
TTGACGTGACCTCAAA28850.1315880695916163No Hit
ATAGGAAACAGACTTC28770.13122318066380595No Hit
AACGTCCCTTACAGAA28610.1304934028081852No Hit
GATACCATGTCTGGAT28570.13031095834428No Hit
CTGCCATTGCACAGTT28280.1289882359809674No Hit
AGCTCGGACCTTAAGC28200.12862334705315703No Hit
TGGAGTTACAAGTCTC28160.12844090258925184No Hit
ATAGCCAACATGGCCC27950.1274830691537496No Hit
GGGATCAGATGAGTAA27670.12620595790641329No Hit
TGTTTGTTGCGCTCGA27400.12497445777505327No Hit
CTGATTACTGCTTTGA27120.12369734652771695No Hit
CGCTGAGTGTTAGCTG26820.12232901304842807No Hit
CCTACGGGATGGAACG26800.12223779081647547No Hit
TTGTAACCTCATCCTC26670.12164484630878361No Hit
CGCTTACTGGCCACGT26580.12123434626499693No Hit
CGATCAAACCGATCGC25950.11836084595849022No Hit
TCAGGCTCTTAAAGCA25880.11804156814665616No Hit
ATCTTACTGCGCACTG25650.11699251247920134No Hit
TCCAAAGTGAAGGCCA25230.11507684560819685No Hit
AGCCGGTACGCGCAAA25230.11507684560819685No Hit
CTCGGATCTTTCTGAG25040.11421023440464721No Hit
CATGTTACTAACGTGG24980.11393656770878945No Hit
TGGGTGCACTTCTGAG24700.11265945646145314No Hit
TTCTAACACTACGTTG24660.11247701199754793No Hit
CGCTTCCCTGCGCTGT24560.11202090083778497No Hit
ACCTGCTTGCCAAACT24160.1101964561987331No Hit
CCTCCCTTGTTTCGCC24110.10996840061885162No Hit
CCTTCAGTGTAGCTTG24050.10969473392299384No Hit
TACAACCGATTCCATT23960.10928423387920717No Hit
TACGCCTTGGGTTGAG23930.10914740053127828No Hit
CTTAGGCCTTATGACC23910.10905617829932569No Hit
GTAAGAAACGACAAAT23850.1087825116034679No Hit
GCATAGTACGCTCCTT23680.10800712263187084No Hit
AGTGGTACTCAACGCC23650.10787028928394198No Hit
TGAGATAGACTCATCC23610.10768784482003678No Hit
ATCGCAATGTAACTCG23510.1072317336602738No Hit
GTGTACTGAATCACAC23340.10645634468867678No Hit
GTCCTGACTTTACGAC23210.1058634001809849No Hit
ATTGAGAGATGATCAC23170.10568095571707972No Hit
AACCACTGAAAACATG23120.10545290013719824No Hit
GAAAGTAACCCTGCGA23000.10490556674548268No Hit
AACAGCTACTCGAGAG22980.10481434451353008No Hit
ACCTCCATGGAATTGA22930.1045862889336486No Hit
CATGTCAGAACAGGTG22900.1044494555857197No Hit
GCATATCGAGCCGCTA22900.1044494555857197No Hit
AGAACAATGAGTCGGA22880.10435823335376712No Hit
TTCTATGACCAACGCC22860.10426701112181452No Hit
TGGTCCTTGTTTCTGT22840.10417578888986194No Hit
GCATGGGCTATGTGGG22740.10371967773009896No Hit
CGAGTCTCTTACTTTC22520.10271623317862043No Hit
TTCACGCTGTGGGCAA22390.10212328867092856No Hit
CCTTCTTTGGATCATC22370.10203206643897597No Hit
GTTGCTAGACGCTCGA22210.10130228858335523No Hit
AGGTTACTGGTCCCGA22090.10075495519163967No Hit
TATCTCCCTGGAATTA21990.1002988440318767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)