FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3409341
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC331420797.20960736987001Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC217800.6388331352012017No Hit
TGTAGATA172130.5048776288438147No Hit
AGTAGATC61710.18100272164034045No Hit
TGTAGAAC61320.17985880555802428Illumina DpnII expression Adapter 1 (100% over 8bp)
TGAAGATC57300.16806767055568803No Hit
TGTACATC53230.15612987964536254No Hit
TCTAGATC39580.11609281676429549ABI Dynabead EcoP Oligo (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)