FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3368798
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA328445097.49619894098726No Hit
GGCAAGGC105580.3134055529598391No Hit
GGCAAGAA105490.3131383953564446No Hit
GGCAACGA81800.24281657730739567No Hit
GGCAAGCA67610.20069472850553818Clontech Universal Primer Mix Long (100% over 8bp)
GCCAAGGA54340.16130382409393498RNA PCR Primer, Index 1 (100% over 8bp)
CGCAAGGA50800.15079562502708682No Hit
GGAAAGGA50010.14845057495284666No Hit
GGCAGGGA47890.14215752918399974No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)