FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19677858
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245690.12485606919208382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG21300.08.7392575
ATACCGT4700.08.6369226
TAGACTG23000.08.4584245
GTGTAGG30300.08.312311
CTATACT21600.08.2270694
TACACCG9700.08.0801375
GTTCGAC6250.08.06520216
GTCCTAG25150.07.90027481
CTAGGAC29100.07.88500173
TACACTG32500.07.8807985
TAGGACT23450.07.81670334
GTATAGA15450.07.78864341
CCTAGAC24650.07.66245273
TAGACAG29850.07.642685
GTGTAGA25550.07.61229751
ATACTGT28750.07.4979366
GTGTATA37750.07.48731761
TACGACG3208.608604E-77.435355
CTAGACT23900.07.43534234
GTCTAGA21100.07.427241
Produced by FastQC (version 0.11.5)