FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20423124
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG9850.08.9542445
TACACTG34350.08.92569355
TATACTG22350.08.5815875
TAGACAG32300.08.2785725
TAGACTG25500.08.2352295
GTCTAAG20850.08.1934671
CTAGGAC29500.08.1631863
CTTAGAC19300.08.1248253
TGGACCG13200.08.0605425
ACACCGT11800.07.94908956
CCTACAC31300.07.87266253
TAGGACT26250.07.83993774
CTACACT32900.07.8297254
TAGGACC22400.07.68743854
TAGACCG8200.07.6828665
GTATTAG21400.07.65571931
GTGTAGG29100.07.6509971
GTATAGA18250.07.51929471
TCTATAC17850.07.45141363
GTATACG4902.5465852E-117.4300411
Produced by FastQC (version 0.11.5)