FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3977472
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT411731.035154992920126No Hit
TGCTGCTACCGTTCTC403631.014790298963764No Hit
AAACGACCTCTTCAAG402481.01189901525391No Hit
ATGGGTTACAATCGAA390970.9829610365578941No Hit
TATGCTTTGGACTGTT381070.9580708550556736No Hit
AAAGCGGACTCGGGCT352870.8871715501705606No Hit
GCAGGTCTGGCTGTTA338550.8511687825835104No Hit
TCCAAAGTGCTCACGG337190.8477495253266396No Hit
GCCTCTTGATCTGTGA335540.8436011617429361No Hit
TAGTGCATGAACCCGC314670.7911306478084572No Hit
TAACTCACTGGTGTCC299000.7517337645620132No Hit
TGTGGCTCTTACGATC298680.7509292334427496No Hit
GAAGTATGAACAGGTG291130.7319473273476218No Hit
TTCCCATCTCTTTGTT269430.6773900608225526No Hit
TCACCTGACTCAATGC258780.6506142595095579No Hit
TGAGAATGAGCAGTTT249790.6280119633777435No Hit
GGAACTACTACCAAGG243880.6131532792688421No Hit
ATGTCCAGAGTCGGTC235300.5915817886335843No Hit
CTAGAGACTAATCTGC233040.5858997876037845No Hit
TCGTATACTGCTAATC232710.5850701148870439No Hit
TTCGTCCGACCTGTGG230820.5803183529638927No Hit
GAGCCTGACCGAGTTT230700.5800166537941688No Hit
TCACACAGATTGGGAG230500.579513821844629No Hit
TGCGGTGACCTTAGTG229670.5774270692540387No Hit
GAAGTAATGAGTCTGT226360.5691052004891549No Hit
ATTCCTAACTGCTACA224420.5642277305786187No Hit
TCCGCGAGAGAACAGC222400.5591491278882668No Hit
AGGTCGAGAAGGGAGG221770.5575652072472164No Hit
TGGACCTCTAGGGAGG214010.5380553276050718No Hit
ACTGATTCTCGGCTCA211880.5327001673424728No Hit
CCATGATGATTCGGGC206610.5194505454720989No Hit
TGGCCTGGACCCGAGT205390.516383270579906No Hit
GCAATCATGGAACGTG186520.46894107614082514No Hit
TTCGATGGAAATCCTG185030.4651949781167536No Hit
ACGAGGATGGAGCTGA183170.4605186409860333No Hit
AAGGTGCGAACAGATA175960.44239154920512325No Hit
TTTCGTAACAGTACGG175100.4402293718221021No Hit
TGGTCCTTGGAGAGGG174240.4380671944390809No Hit
TCCCAGACTGTCTTGA173740.4368101145652314No Hit
TCTCTCTACTCTGGAT165960.41724995172813284No Hit
TTCGATGGACAGGTGA146150.3674444471262148No Hit
TCGCCGAGAAGGTAAT145990.367042181566583No Hit
TGATATCCTCGATGTT145320.3653576945356246No Hit
AGCTGCTCTTGTCACA144770.36397490667439014No Hit
TCCTTCACTTGTCACA143600.3610333397695823No Hit
AGCCCAGTGAAAGTAC141120.3547982235952886No Hit
AACTCCCTGCTTCACC138990.3494430633326897No Hit
GCGGTTCGAGTACGTT137920.3467529124026517No Hit
CTTTCGGTGCTTTGTT137830.3465266380253588No Hit
AACCCTCACGCGGTTC135110.3396881235116174No Hit
CCCTTGCCTCCTACGG131340.33020974126279207No Hit
ATGGCCGTGATACGCT128630.32339636834652763No Hit
TCGGCTTACCTCCTAC126970.3192228631653472No Hit
AAACACGCTGCAGTTT120890.3039367718993371No Hit
TGTTTGTTGCAAGTTC120840.3038110639119521No Hit
GGGGGGGGGGGGGGGG120060.3018500193087469No Hit
ACCCTTGTGGGCCATC116730.29347786734890907No Hit
TGGCCTTTGCGAAAGC116600.2931510265817082No Hit
CTAACTGGAACCTTGA114310.2873936007594774No Hit
TACAACGTGAGCAGGT112350.28246584765398725No Hit
TCAAAGGCTCTTAGTG112150.28196301570444743No Hit
CAATGTGTGGTTGACT111510.28035395346592007No Hit
AAATAGGACTCTATCT110950.2789460240072086No Hit
ACAGCCGACAAACATG105930.2663249420737594No Hit
CTTTCGGTGAGCCATT105630.26557069414944967No Hit
TGAACTGTGTGCTGGC104240.262076012100148No Hit
GGACATTTGGCCGCTA97170.2443009026839158No Hit
AGTGTTCGATTCGCCT97090.2440997699040999No Hit
CAGCCGATGTAATAGG94330.23716068900045054No Hit
GAGGTTGGAGGAATAT89580.2252184301988801No Hit
TTGGGAGGAATCTGTG82820.2082227103044346No Hit
CGACGTTCTTGCTACA81480.20485373624251785No Hit
TGTACTGGAAATGCTC80090.2013590541932162No Hit
GTGCTAATGGGAGTCA78220.196657575465019No Hit
AGGGACCCTAATCCTG77470.1947719556542447No Hit
GGAACATCTTTACGAC76060.19122699040998906No Hit
GGGTGAAACCGACTAT75310.18934137059921477No Hit
TCTTGAACTCGTACAT74510.18733004280105553No Hit
TACGTGTGAAACGCAG74490.18727975960610158No Hit
GGACCACTGGCTAGAG73750.18541928139280428No Hit
TACCACCTGAAACATG73020.18358394477698398No Hit
AGCTTGTGAACCGTGC72530.18235200650061142No Hit
TAACGAGCTTACTTTC72160.1814217673939628No Hit
CGGATGAGAACAGGCA71880.18071780266460707No Hit
ACTTATCTGTCAGGTT69920.17579004955911695No Hit
CATGATGTGCCTAGTA67470.1696303581772543No Hit
ACGTGTTGATCTTTGT63460.15954857758898114No Hit
TCGGACCGAATGTCCA62690.15761267458325287No Hit
AACTACGACATTGCCA62640.15748696659586792No Hit
TGGTAGGGATGTGCTA62440.15698413464632813No Hit
AACTAGCACTGCCACG61720.1551739396279848No Hit
CGTCGTACTTGCGCCA60270.1515284079938212No Hit
AACGAACCTGACACCC59270.14901424824612217No Hit
TACCACCTGTACTTTC58960.14823485872433545No Hit
GTAGATAGAGCTGAGA58860.14798344274956557No Hit
CCTTCAGTGCAACGCC58440.14692749565553195No Hit
CTCGGATCTAGTACGG58330.14665093808328505No Hit
TTTAGGGACTGGGCAA57860.1454692830018665No Hit
GTCCGAGTGGTCTTGA57630.14489102625989575No Hit
AGTTTGCGAGCAGGAT57050.14343281360623028No Hit
GGTAGTTTGTCCAGCG56250.14142148580807107No Hit
TGAGGAGTGGTCCCAG55930.14061695468880736No Hit
CTTTAACTGTTCGCCT54560.13717255583445967No Hit
GCCATAATGACCGTTA54160.13616689193538006No Hit
GGCTTGCGAATACGCT53730.13508580324386946No Hit
AGAACGCGACACAGTT51890.13045974930810325No Hit
GGGATGTGATGCATGT50250.1263365273218768No Hit
CTTACATTGGAAGGCT50040.12580855377486003No Hit
CCGTTACTGTTGGTTG49610.12472746508334943No Hit
GTCCGTTACGGATGGT49300.12394807556156272No Hit
CAGGCCATGTCGTAGT48970.12311840284482203No Hit
CGATCGGTGCGAGGAT48720.12248986290789728No Hit
TGCAATGACTCCGTAA48170.12110707504666282No Hit
CAAAGACCTGCTTTGA48150.12105679185170883No Hit
AACTACGACGCTGTTA47240.1187689064813027No Hit
GAGAAGAGATGCTACA46280.11635531312351162No Hit
TCCTCATCTTTCTGGA46280.11635531312351162No Hit
GAACCATACTTTGGGT46210.11617932194117268No Hit
AATTCTGCTTAGCGAG45270.11381601177833559No Hit
TGTCTTCCTGAAATCA44660.11228237433223917No Hit
GAAAGTCCTTATCTGG44000.11062302889875779No Hit
CCCACCTCTTGACCTA44000.11062302889875779No Hit
CCTGAGGACTGACTCA43920.11042189611894188No Hit
TCCGTAAGATTACGCA43590.10959222340220119No Hit
ATGGCCGTGGGCCAAA43380.10906424985518441No Hit
TCGTAGAACCGCACTG42940.10795801956619683No Hit
GAAGGACACCCAGCTG42230.1061729661453305No Hit
TATGGGTACCCTTTGC41930.1054187182210208No Hit
GATAAGAACATTGGTC41040.10318111604556864No Hit
ACAAGACCTAAGCTAA40160.10096865546759348No Hit
AGGTGATCTGTTAGTC39940.10041554032309971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)