FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594094
Sequences flagged as poor quality0
Sequence length8
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCAA149830293.99081860919118No Hit
TTAGTCAA709514.450866761934993No Hit
TCAGACAA40500.2540628093449947No Hit
TCAGGCAA33160.20801784587358088No Hit
ACAGTCAA32580.20437941551752908TruSeq Adapter, Index 13 (100% over 8bp)
TCAGTAAA31700.19885903842558847No Hit
TCAGTCAT17930.11247768324829026No Hit
TAAGTCAA17790.11159944143820878No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)