FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15498940
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT5150.010.0582246
GTATTAG15700.09.0084071
GTCCTAG21750.08.5628571
CTAGTAC8350.08.5513423
GAACCGT6400.08.5312256
TAGACAG25950.08.362215
TCTATAC13400.08.3586443
TAGACTG19000.08.3262915
CTAGGAC22900.08.3148423
TACACTG26550.08.2786965
TTATACT17800.08.2584044
GTACTAG11800.08.18829251
TACACAG34650.07.95957335
TATACTG19200.07.9478935
TAGAACC16450.07.91487034
TAGGACC15950.07.8996644
CCTACAC22450.07.857873
TACACCG6950.07.8560935
GTGTAGG24050.07.80218121
TCTACAC18100.07.73520853
Produced by FastQC (version 0.11.5)