FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453272
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT238875197.37000218483723No Hit
TCACTCCA150070.6117136624067775No Hit
ACACTCCT77180.3146002563107556No Hit
GCACTCCT54790.22333438770751876No Hit
TCCCTCCT53450.21787229463345278No Hit
TCACACCT40820.1663900293159503No Hit
TCACTCAT36550.14898470287844154RNA PCR Primer, Index 45 (100% over 8bp)
TCACTACT29000.11820947697605483No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)