FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453272
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT440301.794745955605412No Hit
GAAGCAGTGGAACCTT154660.6304233692798842No Hit
GTTCTTCCTAACGTAA129360.5272957910904295No Hit
CGCTTAAGATAATAGG104200.42473887934154875No Hit
CATGGAGGATCGACAT103370.4213556425867168No Hit
GATACGTACCGCTCGA99880.40712974346097786No Hit
TGTCCCATGAGACAAT97860.3988958419612664No Hit
AAAGCATGATCTTTGT96370.39282232055801397No Hit
TGAGCGCACAGGACAC88710.3615987138808905No Hit
TAATTGCCTGTCTGGC82880.33783453281984227No Hit
TGTCGAAACTTCGCAA81610.3326577729660633No Hit
GGGGGGGGGGGGGGGG76650.3124398762142967No Hit
GGAAGGACTAGGTAAT72090.2938524550070274No Hit
CATGTCAGAGGTTTGT66930.27281932048301205No Hit
AATGATGGAAAGCTAA66330.27037360716626613No Hit
TCGACCTTGTTTCGCC61540.2508486625209108No Hit
GTGAGATCTCGATTAC53150.21664943797507982No Hit
ATGGGTACTTGGCTGC52170.2126547728910614No Hit
AAACGACCTAGGGAGG49310.20099687274790565No Hit
AGCCTACCTTAACTCG47560.1938635422407299No Hit
TTGCGATGACATTCAG46590.18990963904532396No Hit
GCTATGATGACCTGCG46450.18933897260474988No Hit
TGTTCCTCTTTATGGC45540.18562964074101854No Hit
CAGCTCACTGTTTACG44350.18077897599613904No Hit
CTGCCTTGACCATAAC43810.17857783401106767No Hit
CCTGTTGACCCTAGTA42960.17511307347901087No Hit
CAATGTGACCAACGCC42250.17221897938752817No Hit
TAACCAGACATCACAC41520.16924336151882058No Hit
CATGATGTGTCATGTG40590.16545250587786434No Hit
CATGCAAACGAAGGCT40150.1636589827789173No Hit
CACAGCTCTGGACTGA39950.16284374500666865No Hit
TGGGCCATGCGTTCTC39300.16019422224686053No Hit
CTATTCAACCGAACGA39130.15950127014044915No Hit
ATGCCGCGATGCCACG39130.15950127014044915No Hit
ATGGGTACTTTCGGGC38450.15672946171480373No Hit
TTGTTTGTGATACGCT38220.15579193827671778No Hit
AGAACGCGAGGAATAT37790.1540391770663832No Hit
ATTAGCGTGGTCGGTC37570.15314241551690966No Hit
CATTAGTCTATTCTGG37070.15110432108628802No Hit
AGCTTGTGACTCACTC36730.14971841687346532No Hit
CCGCTATTGTTCGCAA35370.14417480002217445No Hit
CACTATACTACGCCAC34820.14193289614849067No Hit
GGGCTGACTAATACAG34530.1407508013787301No Hit
AACGTCCCTTACAGAA33910.1382235642847593No Hit
AACCAACACCGGAATG33780.13769365973279765No Hit
GGTAGCCTGTGCCACG33410.13618546985413765No Hit
ATAGGAAACAGACTTC33270.1356148034135636No Hit
TTGACGTGACCTCAAA33260.13557404152495117No Hit
AGCTCGGACCTTAAGC32750.1334951852057171No Hit
TGGAGTTACAAGTCTC32330.13178318588399493No Hit
GCAGATACTCGGCTCA32160.13109023377758358No Hit
TTGTAACCTCATCCTC31970.13031575789394734No Hit
CCCAATGCTGGAATGC31950.1302342341167225No Hit
CTGATTACTGCTTTGA31640.12897061556973707No Hit
GCATCTCTGAGCCTAT31640.12897061556973707No Hit
CTGCCATTGCACAGTT31520.12848147290638787No Hit
CCTACGGGATGGAACG31420.12807385402026356No Hit
TGTTTGTTGCGCTCGA31000.1263618546985414No Hit
CGCTGAGTGTTAGCTG30940.12611728336686678No Hit
ATCTTACTGCGCACTG30760.125383569371843No Hit
GGGATCAGATGAGTAA30720.12522052181739327No Hit
CGCTTACTGGCCACGT30700.1251389980401684No Hit
ATAGCCAACATGGCCC30570.12460909348820676No Hit
TCAGGCTCTTAAAGCA30480.12424223649069488No Hit
CGATCAAACCGATCGC30440.12407918893624514No Hit
GATACCATGTCTGGAT29740.12122585673337485No Hit
CGCTTCCCTGCGCTGT29660.12089976162447538No Hit
TCCAAAGTGAAGGCCA28970.11808719131021755No Hit
CTCGGATCTTTCTGAG28910.11784261997854295No Hit
AGCCGGTACGCGCAAA28500.1161713825454332No Hit
TTCTAACACTACGTTG28430.11588604932514616No Hit
TGGGTGCACTTCTGAG28340.11551919232763429No Hit
CGAGTCTCTTACTTTC28320.11543766855040942No Hit
CATGTTACTAACGTGG28000.11413328811481158No Hit
AGTGGTACTCAACGCC27800.11331805034256293No Hit
CCTTCAGTGTAGCTTG27680.1128289076792137No Hit
CTTAGGCCTTATGACC27530.11221747935002722No Hit
ACCTGCTTGCCAAACT27440.11185062235251533No Hit
CCTCCCTTGTTTCGCC27350.11148376535500344No Hit
TACAACCGATTCCATT27350.11148376535500344No Hit
AACAGCTACTCGAGAG27290.11123919402332885No Hit
ATTGAGAGATGATCAC27230.11099462269165425No Hit
AGAACAATGAGTCGGA27210.11091309891442938No Hit
ATCGCAATGTAACTCG27160.11070928947136721No Hit
GTCCTGACTTTACGAC27080.11038319436246775No Hit
TGGTCCTTGTTTCTGT26920.10973100414466883No Hit
TACGCCTTGGGTTGAG26850.10944567092438182No Hit
GTGTACTGAATCACAC26680.10875271881797045No Hit
TGAGATAGACTCATCC26400.10761138593682233No Hit
AACCACTGAAAACATG26400.10761138593682233No Hit
GCATGGGCTATGTGGG25840.1053287201745261No Hit
GGGTCGTACCGCTCGA25830.10528795828591367No Hit
TATCTCCCTGGAATTA25740.10492110128840178No Hit
TTCTATGACCAACGCC25700.10475805373395206No Hit
CATGTCAGAACAGGTG25690.10471729184533961No Hit
AGGTTACTGGTCCCGA25690.10471729184533961No Hit
GCGTCGATGCTTGAAC25660.10459500617950232No Hit
AGAACATGAAAGCTAA25580.10426891107060286No Hit
ACCTCCATGGAATTGA25360.10337214952112932No Hit
TTCACGCTGTGGGCAA25340.10329062574390446No Hit
GTAAGAAACGACAAAT25340.10329062574390446No Hit
TCGACCTTGGGACCTC25220.10280148308055527No Hit
GCATAGTACGCTCCTT25190.10267919741471798No Hit
GTTGCTAGACGCTCGA25110.10235310230581852No Hit
CCTTCTTTGGATCATC25070.10219005475136879No Hit
TCCGGGATGCGAGTTT25050.10210853097414392No Hit
AAAGGATACTGCTGGC25020.10198624530830662No Hit
GAAAGTAACCCTGCGA24670.10055957920687149No Hit
AGAGAATTGCCCGCAA24660.10051881731825904No Hit
ATTTCTGACCATCCTC24650.10047805542964662No Hit
TCAGCGCGATCCAGCG24640.10043729354103417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)