FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18338760
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC19900.012.02902122
CGAGACA22150.011.0599123
CCCACGA23250.010.41616919
CTTATAC26800.010.34682751
ATCTCCG24250.010.217792510
TATACAC38950.010.0313753
TCTCCGA25250.09.813127511
CACGAGA25250.09.75747321
GTATACG3703.6379788E-129.0841911
TAGGACA23950.08.770964
TAGACTG26350.08.7162195
TTATACA36650.08.6357132
CCACGAG29400.08.61816920
TACACTG33500.08.4862565
TATACTG23900.08.379215
TAGACAG33150.08.3223815
TCTATAC17050.08.2958473
TAGGACG7200.08.1691744
TACACAT43550.08.1679015
CTAGGAC28900.08.0924943
Produced by FastQC (version 0.11.5)