FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3923788
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT401921.0243162984340641No Hit
AAACGACCTCTTCAAG397631.0133829860328845No Hit
TGCTGCTACCGTTCTC397131.0121087071982482No Hit
ATGGGTTACAATCGAA389430.9924848131448487No Hit
TATGCTTTGGACTGTT379780.9678912316363678No Hit
AAAGCGGACTCGGGCT351490.8957925351726443No Hit
TCCAAAGTGCTCACGG330090.8412534010502096No Hit
GCAGGTCTGGCTGTTA328940.8383225597305461No Hit
GCCTCTTGATCTGTGA324590.82723633386921No Hit
TAGTGCATGAACCCGC310710.7918623534197056No Hit
TGTGGCTCTTACGATC294010.7493014403428523No Hit
TAACTCACTGGTGTCC292420.7452492336487089No Hit
GAAGTATGAACAGGTG280690.7153546521881406No Hit
TTCCCATCTCTTTGTT269680.6872950322494488No Hit
TCACCTGACTCAATGC259460.6612487728694822No Hit
TGAGAATGAGCAGTTT247130.6298250568073505No Hit
GGAACTACTACCAAGG234970.598834595548995No Hit
ATGTCCAGAGTCGGTC231640.5903478985103171No Hit
TCGTATACTGCTAATC231120.5890226485222954No Hit
CTAGAGACTAATCTGC229220.5841803889506773No Hit
TTCGTCCGACCTGTGG229050.5837471341469008No Hit
ATTCCTAACTGCTACA227400.579542013992601No Hit
TGCGGTGACCTTAGTG225330.5742664996172067No Hit
GAGCCTGACCGAGTTT223180.5687871006282704No Hit
TCACACAGATTGGGAG221760.5651681487379032No Hit
AGGTCGAGAAGGGAGG220010.5607081728166761No Hit
TCCGCGAGAGAACAGC219570.5595868074421962No Hit
GAAGTAATGAGTCTGT217290.5537760959562545No Hit
ACTGATTCTCGGCTCA214830.5475066440898438No Hit
TGGACCTCTAGGGAGG208910.5324191826877497No Hit
TGGCCTGGACCCGAGT204780.5218936395136536No Hit
CCATGATGATTCGGGC204670.5216132981700337No Hit
TTCGATGGAAATCCTG186290.4747708082088023No Hit
GCAATCATGGAACGTG181050.46141636602181363No Hit
ACGAGGATGGAGCTGA178110.4539236064741521No Hit
AAGGTGCGAACAGATA177140.4514515055349576No Hit
TTTCGTAACAGTACGG174390.44444297194445775No Hit
TGGTCCTTGGAGAGGG169870.43292349127934543No Hit
TCCCAGACTGTCTTGA169180.43116498648754725No Hit
TCTCTCTACTCTGGAT166870.4252778182715274No Hit
TTCGATGGACAGGTGA147490.3758867706410234No Hit
TGATATCCTCGATGTT145720.3713758235664108No Hit
AGCTGCTCTTGTCACA143970.3669158476451837No Hit
TCGCCGAGAAGGTAAT143470.3656415688105474No Hit
AACTCCCTGCTTCACC140990.35932114579075114No Hit
TCCTTCACTTGTCACA139730.3561099631274677No Hit
AGCCCAGTGAAAGTAC139510.35554928044022766No Hit
CTTTCGGTGCTTTGTT137560.350579592985146No Hit
AACCCTCACGCGGTTC133510.34025793442459173No Hit
GCGGTTCGAGTACGTT130210.33184769411599196No Hit
CCCTTGCCTCCTACGG130000.33131249700544474No Hit
ATGGCCGTGATACGCT129330.3296049633670321No Hit
TCGGCTTACCTCCTAC128560.3276425739616921No Hit
GGGGGGGGGGGGGGGG122960.31337065101376527No Hit
AAACACGCTGCAGTTT121990.3108985500745708No Hit
TGTTTGTTGCAAGTTC120180.3062856606931873No Hit
ACCCTTGTGGGCCATC116490.2968814828935712No Hit
TGGCCTTTGCGAAAGC114580.29201373774526046No Hit
CAATGTGTGGTTGACT112800.28747730509395514No Hit
AAATAGGACTCTATCT112250.28607559837585517No Hit
CTAACTGGAACCTTGA111280.2836034974366607No Hit
TACAACGTGAGCAGGT111180.28334864166973345No Hit
TCAAAGGCTCTTAGTG105330.2684395793044884No Hit
TGAACTGTGTGCTGGC104940.2674456418134721No Hit
ACAGCCGACAAACATG104750.2669614158563103No Hit
CTTTCGGTGAGCCATT103490.26375023319302676No Hit
AGTGTTCGATTCGCCT97420.24828048814054174No Hit
CAGCCGATGTAATAGG93940.2394115074514729No Hit
GGACATTTGGCCGCTA92630.23607289690472572No Hit
GAGGTTGGAGGAATAT83790.2135436471083555No Hit
CGACGTTCTTGCTACA81870.208650416383352No Hit
TTGGGAGGAATCTGTG81720.20826813273296113No Hit
TGTACTGGAAATGCTC79850.20350232989142122No Hit
AGGGACCCTAATCCTG76870.19590762803698875No Hit
GTGCTAATGGGAGTCA76760.19562728669336873No Hit
GGAACATCTTTACGAC73930.18841486848932715No Hit
TAACGAGCTTACTTTC73780.18803258483893623No Hit
CGGATGAGAACAGGCA73110.1863250512005236No Hit
TACGTGTGAAACGCAG72600.18502528678919453No Hit
GGGTGAAACCGACTAT72040.18359809449440184No Hit
TACCACCTGAAACATG71920.18329226757408912No Hit
GGACCACTGGCTAGAG70930.1807691954815092No Hit
TCTTGAACTCGTACAT70540.17977525799049288No Hit
ACTTATCTGTCAGGTT69190.17633470513697477No Hit
AGCTTGTGAACCGTGC67930.17312352247369125No Hit
CATGATGTGCCTAGTA67860.17294512343684215No Hit
ACGTGTTGATCTTTGT61840.15760280626782078No Hit
AACTAGCACTGCCACG61730.1573224649242008No Hit
TGGTAGGGATGTGCTA61230.15604818608956447No Hit
AACTACGACATTGCCA60490.1541622534143027No Hit
CGTCGTACTTGCGCCA59170.15079815729086282No Hit
AACGAACCTGACACCC58980.15031393133370102No Hit
CCTTCAGTGCAACGCC58320.14863188327198107No Hit
TCGGACCGAATGTCCA58100.14807120058474108No Hit
TACCACCTGTACTTTC58080.14802022943135562No Hit
CTCGGATCTAGTACGG57910.14758697462757928No Hit
GTCCGAGTGGTCTTGA57520.14659303713656294No Hit
TTTAGGGACTGGGCAA57330.14610881117940114No Hit
AGTTTGCGAGCAGGAT56180.1431779698597376No Hit
TGAGGAGTGGTCCCAG56100.14297408524619576No Hit
GTAGATAGAGCTGAGA56010.14274471505596123No Hit
GGTAGTTTGTCCAGCG54240.13823376798134862No Hit
GCCATAATGACCGTTA52770.13448738820751785No Hit
CTTTAACTGTTCGCCT51480.1311997488141561No Hit
AGAACGCGACACAGTT51170.13040969593668159No Hit
GGCTTGCGAATACGCT50390.12842182095464894No Hit
CTTACATTGGAAGGCT50140.12778468153733077No Hit
CAAAGACCTGCTTTGA49070.12505772483120903No Hit
TGCAATGACTCCGTAA48970.12480286906428177No Hit
CGATCGGTGCGAGGAT48710.12414024407027087No Hit
GTCCGTTACGGATGGT48670.12403830176349996No Hit
CCGTTACTGTTGGTTG48260.12299339311909818No Hit
GGGATGTGATGCATGT47500.12105648929045096No Hit
AATTCTGCTTAGCGAG46830.11934895565203828No Hit
CAGGCCATGTCGTAGT46030.11731010951662017No Hit
GAGAAGAGATGCTACA45970.1171571960564638No Hit
TCCTCATCTTTCTGGA45660.11636714317898929No Hit
GAACCATACTTTGGGT45510.1159848595285984No Hit
CCCACCTCTTGACCTA45430.11578097491505657No Hit
AACTACGACGCTGTTA44890.11440475377364934No Hit
GAAAGTCCTTATCTGG43870.11180522495099124No Hit
CCTGAGGACTGACTCA43540.11096420092013126No Hit
TGTCTTCCTGAAATCA42860.10923118170502585No Hit
ATGGCCGTGGGCCAAA42290.10777850383354044No Hit
TCCGTAAGATTACGCA41980.10698845095606592No Hit
TCGTAGAACCGCACTG41820.10658068172898229No Hit
GATAAGAACATTGGTC40790.10395566732963146No Hit
TATGGGTACCCTTTGC40690.1037008115627042No Hit
AGGTGATCTGTTAGTC39770.10135613850697335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)